GFP Antikörper (Green Fluorescent Protein) (Atto 488)

Details for Product anti-GFP Antibody No. ABIN4368303, Anbieter: Anmelden zum Anzeigen
Antigen
  • green fluorescent protein
  • gfp
Reaktivität
Aequorea victoria
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Wirt
Camelid (Camelidae)
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Antikörpertyp
Recombinant Antibody
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser GFP Antikörper ist konjugiert mit Atto 488
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Applikation
Immunocytochemistry (ICC), Immunofluorescence (IF)
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Optionen
Hersteller
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Hersteller Produkt- Nr.
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Verwendungszweck FluoTag-X series - Multiple fluorophores per affinity probe and multiple affinity probes per target molecule resulte in maximal brightness
Marke FluoTag-X
Immunogen GFP
Klon 1H1-1B2
Fragment single-domain Antibody (sdAb)
Spezifität GFP, mEGFP, sfGFP and most common CFP and YFP variants
Kreuzreaktivität (Details) Does not cross-react with mCherry, mRFP, dsRed, mTagBFP or their most common derivatives
Produktmerkmale FluoTag-X4 anti-GFP is a blend of two in-house developed single-domain antibodies (sdAbs) that recognize two distinct epitopes on GFP and its most common derivatives with high affinity and specificity. In our FluoTag-X4 series, two fluorophore molecules are site-specifically coupled to each individual FluoTag molecule. The reagent therefore simultaneously targets up to four fluorophores to the protein of interest, which ensures extra-bright signals. Owing to the small size of our FluoTags, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, our FluoTag-X4 series can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render our FluoTag-X4 products excellent tools for all microscopy techniques.
Andere Bezeichnung GFP
Hintergrund Green fluorescent protein GFP and its derivates have become very popular and universal tools in cell biology. It is a monomeric protein and an excellent fusion partner for chimeric proteins. The possibility to fuse GFP to the N- or the C-terminus of the protein of interest enables a wide range of application. However for some microscopy technics (like STROM or STED) more photostable or blinking fluorophores are needed, but also in conventional immnuofluorescence studies an amplification of the signal or the possible to image at another wavelength might be preferable. With our FluoTags the potential of GFP fusion proteins can be exceeded by far.
Molekulargewicht 27 kDa
Forschungsgebiet Tags/Labels
Applikationshinweise 1:250 (IF,ICC)
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 2.5 μM
Buffer PBS, 50 % glycerol, 0.09 % sodium azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Protect from light!
Lagerung -20 °C
Informationen zur Lagerung Up to 3 months: -20 °C Up to 12 months: -80 °C or below
Bilder des Herstellers
Immunofluorescence (IF) image for anti-Green Fluorescent Protein (GFP) antibody (Atto 488) (ABIN4368303) Stable HeLa cell line expressing YFP-fusion protein in the nuclear pore complex (A, y...
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