MBD2 Antikörper (Internal Region, Isoform 1)
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- Target Alle MBD2 Antikörper anzeigen
- MBD2 (Methyl-CpG Binding Domain Protein 2 (MBD2))
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Bindungsspezifität
- Internal Region, Isoform 1
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Reaktivität
- Human, Maus, Ratte, Hund
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Dieser MBD2 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- Sequenz
- C-RNDPLNQNKG KPDLN
- Spezifität
- This antibody is expected to resognize isoform 1 (NP_003918.1) only.
- Kreuzreaktivität (Details)
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Species reactivity (expected):Mouse, Rat, Canine.
Species reactivity (tested):Human. - Aufreinigung
- Affinity Chromatography
- Immunogen
- Peptide with sequence from the internal region of the protein sequence Genename: MBD2
- Top Product
- Discover our top product MBD2 Primärantikörper
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- Applikationshinweise
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Peptide ELISA: 1/64000. Western blot: Approx 48-50 kDa band observed in lysates of cell line Jurkat (calculated MWof 43.0 kDa according to NP_003918.1). The observed molecular weight corresponds toearlier findings in literature with different antibodies (Tan et al, Mol Cell Biol.2006Oct,26(19): 7224-35. , PMID: 16980624). Recommended concentration: 0.3 - 1 μg/mL. Immunohistochemistry on paraffin sections: Human Colon shows nuclear stianing ofenterocytes. Recommended concentration: 2 - 4 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 0.5 mg/mL
- Buffer
- Tris saline, 0.02 % Sodium Azide, pH 7.3 with 0.5 % BSA
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- MBD2 (Methyl-CpG Binding Domain Protein 2 (MBD2))
- Andere Bezeichnung
- MBD2 (MBD2 Produkte)
- Synonyme
- MBD2 antikoerper, DMTase antikoerper, NY-CO-41 antikoerper, MBD2a antikoerper, wu:fa97f06 antikoerper, zgc:111862 antikoerper, methyl-CpG binding domain protein 2 antikoerper, methyl-CpG binding domain protein 2 S homeolog antikoerper, MBD2 antikoerper, Mbd2 antikoerper, mbd2 antikoerper, mbd2.S antikoerper
- Hintergrund
- DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. This protein may function as a mediator of the biological consequences of the methylation signal.Synonyms: DMTase, Demethylase, Methyl-CpG-binding domain protein 2
- Gen-ID
- 8932
- NCBI Accession
- NP_003918
- UniProt
- Q9UBB5
- Pathways
- Chromatin Binding
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