MBD2 Antikörper (Internal Region, Isoform 1)

Produktnummer ABIN375214

Produktdetails anti-MBD2 Antikörper

Target: MBD2 (Methyl-CpG Binding Domain Protein 2 (MBD2))

Bindungsspezifität: Internal Region, Isoform 1

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser MBD2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequenz: C-RNDPLNQNKG KPDLN

Spezifität: This antibody is expected to resognize isoform 1 (NP_003918.1) only.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Canine.
Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography

Immunogen: Peptide with sequence from the internal region of the protein sequence Genename: MBD2

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/64000. Western blot: Approx 48-50 kDa band observed in lysates of cell line Jurkat (calculated MWof 43.0 kDa according to NP_003918.1). The observed molecular weight corresponds toearlier findings in literature with different antibodies (Tan et al, Mol Cell Biol.2006Oct,26(19): 7224-35. , PMID: 16980624). Recommended concentration: 0.3 - 1 μg/mL. Immunohistochemistry on paraffin sections: Human Colon shows nuclear stianing ofenterocytes. Recommended concentration: 2 - 4 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % Sodium Azide, pH 7.3 with 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu MBD2

Target: MBD2 (Methyl-CpG Binding Domain Protein 2 (MBD2))

Andere Bezeichnung: MBD2 (MBD2 Produkte)

Synonyme: MBD2 antikoerper, DMTase antikoerper, NY-CO-41 antikoerper, MBD2a antikoerper, wu:fa97f06 antikoerper, zgc:111862 antikoerper, methyl-CpG binding domain protein 2 antikoerper, methyl-CpG binding domain protein 2 S homeolog antikoerper, MBD2 antikoerper, Mbd2 antikoerper, mbd2 antikoerper, mbd2.S antikoerper

Hintergrund: DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. This protein may function as a mediator of the biological consequences of the methylation signal.Synonyms: DMTase, Demethylase, Methyl-CpG-binding domain protein 2

Gen-ID: 8932

NCBI Accession: NP_003918

UniProt: Q9UBB5

Pathways: Chromatin Binding