GFAP Antikörper (C-Term)

Produktnummer ABIN374749

Produktdetails anti-GFAP Antikörper

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Bindungsspezifität: C-Term

Reaktivität: Human, Ratte, Maus, Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser GFAP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequenz: C-DGEVIKESKQ EHKD

Spezifität: This antibody recognizes GFAP.

Kreuzreaktivität (Details): Species reactivity (expected):Rat, Canine.
Species reactivity (tested):Human, Mouse.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Synthetic peptide from the C-Terminus of Human GFAP (NP_002046.1) Genename: GFAP

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Detection Limit: 1/32000. Western Blot: 0.1-0.3 μg/mL. Approx 50 kDa band observed in Human Brain Cerebellum andMouse Brain lysates (calculated MW of 49.0 kDa according to Mouse NP_034407.2 andHuman NP_002046.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Andere Bezeichnung: GFAP (GFAP Produkte)

Synonyme: GFAP antikoerper, AI836096 antikoerper, cb345 antikoerper, etID36982.3 antikoerper, gfapl antikoerper, wu:fb34h11 antikoerper, wu:fk42c12 antikoerper, xx:af506734 antikoerper, zgc:110485 antikoerper, glial fibrillary acidic protein antikoerper, GFAP antikoerper, LOC100136168 antikoerper, gfap antikoerper, Gfap antikoerper

Hintergrund: Glial fibrillary acidic protein (GFAP) is a member of the class III intermediate filament protein family. It is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. GFAP is thought to help to maintain astrocyte mechanical strength, as well as the shape of cells but its exact function remains poorly understood, despite the number of studies using it as a cell marker.GFAP is thought to help to maintain astrocyte mechanical strength, as well as the shape of cells but its exact function remains poorly understood, despite the number of studies using it as a cell marker.Synonyms: Glial Fibrillary Acidic Protein

Gen-ID: 2670

NCBI Accession: NP_001229305

UniProt: P14136