GFP Antikörper (Green Fluorescent Protein)

Details for Product anti-GFP Antibody No. ABIN285794, Anbieter: Anmelden zum Anzeigen
Antigen
  • green fluorescent protein
  • gfp
Reaktivität
Aequorea victoria
638
16
5
5
2
2
Wirt
Kaninchen
340
170
80
40
16
9
9
2
Klonalität
Polyklonal
Konjugat
Dieser GFP Antikörper ist unkonjugiert
33
31
28
14
12
12
12
9
9
8
8
7
7
6
6
6
6
6
5
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Applikation
Immunoprecipitation (IP), Western Blotting (WB)
536
354
202
156
144
134
96
87
73
25
15
13
13
8
8
6
6
5
4
4
3
3
3
3
3
2
2
2
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen GFP antibody was raised in rabbit using E. Coli-expressed full length green fluorescent protein as the immunogen.
Spezifität This antibody neutralizes and binds to human interferon alpha receptor with high affinity. It interacts with extracellular domain and blocks biological action of Type I interferons.
Kreuzreaktivität (Details) This antibody reacts with wild -type GFP and it's variants, such as EGFP, EBFP
Reinigung purified
Andere Bezeichnung GFP
Substanzklasse Tag
Hintergrund The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to blue light. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm. In cell and molecular biology, the GFP gene is frequently used as a reporter of expression.
Forschungsgebiet Tags/Labels
Applikationshinweise IP: 1:200-1:500, WB: 1:5,000
Optimal conditions should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Reconstitue in distilled water.
Konzentration Lot specific
Buffer Supplied as a lyophilized Potein A purified rabbit IgG with 0.1 % NaN3
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handhabung Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.
Lagerung RT/-20 °C
Informationen zur Lagerung Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.
Bilder des Herstellers
Western Blotting (WB) image for anti-Green Fluorescent Protein (GFP) antibody (ABIN285794) Lane 1 - Marker; Lane - GP fusion protein; Lane 3 - Control
Produkt verwendet in: Orozco, Tahirovic, Rentzsch, Schwenk, Haass, Edbauer: "Loss of fused in sarcoma (FUS) promotes pathological Tau splicing." in: EMBO reports, Vol. 13, Issue 8, pp. 759-64, 2012 (PubMed).

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