rho Guanine Nucleotide Exchange Factor (GEF) 4 (ARHGEF4) (AA 143-271) Antikörper

Details zu Produkt Nr. ABIN2749042, Anbieter: Anmelden zum Anzeigen
Antigen
  • ARHGEF4
  • ASEF
  • ASEF1
  • GEF4
  • STM6
  • 9330140K16Rik
  • Asef
  • ENSMUSG00000070955
  • Rho guanine nucleotide exchange factor (GEF) 4
  • Rho guanine nucleotide exchange factor 4
  • ARHGEF4
  • arhg4
  • Arhgef4
Epitop
AA 143-271
13
9
9
8
4
1
1
1
1
Reaktivität
Human
46
2
1
1
1
1
1
1
Wirt
Maus
21
16
9
Klonalität (Klon)
Monoklonal ()
Konjugat
Unkonjugiert
7
3
3
3
3
3
Applikation
Flow Cytometry (FACS), Western Blotting (WB)
42
29
8
2
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Recombinant fragment of human ARHGEF4 (amino acids 143-271)
Klon ARHGEF-08
Isotyp IgG1
Spezifität The mouse monoclonal antibody ARHGEF-08 recognizes human intracellular protein ARHGEF4 / ASEF1, approx. 80 kDa guanine nucleotide exchange factor specific for Rac1 and Cdc42.
Reinigung Purified from cell culture supernatant by protein-A affinity chromatography.
Reinheit > 95 % (by SDS-PAGE)
Andere Bezeichnung ARHGEF4 (ARHGEF4 Antibody Abstract)
Hintergrund ARHGEF4 (Rho guanine nucleotide exchange factor 4), also known as ASEF 1 (adenomatous polyposis coli –, stimulated guanine nucleotide exchange factor 1) is an approximately 80 kDa cytoplasmic protein important for growth factor-mediated regulation of cell morphology and migration. Besides N-terminal adenomatous polyposis coli (APC)-binding region (ABR) it contains Dbl homology (DH), Pleckstrin homology (PH) and SH3 domains. The SH3 domain inhibits GEF activity of ARHGEF4 by intramolecular interaction with the DH domain, whereas binding of APC stimulates the GEF activity. Activated ARHGEF4 stimulates the small GTPase Cdc42, which leads to decreased cell-cell adherence and enhanced cell migration.
Forschungsgebiet Signaling, Proteolysis / Ubiquitin, Cytokines, Growth Factors, Receptors, Immunology, Inflammation
Pathways Neurotrophin Signalübertragung
Applikationshinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Lagerung 4 °C
Informationen zur Lagerung Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Bilder des Herstellers
Western Blotting (WB) image for anti-rho Guanine Nucleotide Exchange Factor (GEF) 4 (ARHGEF4) (AA 143-271) antibody (ABIN2749042) Western blotting analysis of ARHGEF4 in HEK293 cells (A) and HEK293-ARHGEF4 transfect...
Allgemeine Veröffentlichungen Zhang, Chen, Gao, Lin, Zhu, Lu, Shi, Gao, Zhou, Xu, Zhang, Wu: "Structural basis for the recognition of Asef by adenomatous polyposis coli." in: Cell research, Vol. 22, Issue 2, pp. 372-86, 2012 (PubMed).

Lyons, Williams, Morrow, Sebire, Hubank, Anderson: "The RAC specific guanine nucleotide exchange factor Asef functions downstream from TEL-AML1 to promote leukaemic transformation." in: Leukemia research, Vol. 34, Issue 1, pp. 109-15, 2010 (PubMed).

Kawasaki, Tsuji, Sagara, Echizen, Shibata, Akiyama: "Adenomatous polyposis coli and Asef function downstream of hepatocyte growth factor and phosphatidylinositol 3-kinase." in: The Journal of biological chemistry, Vol. 284, Issue 33, pp. 22436-43, 2009 (PubMed).

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