CDC27 Antikörper

Produktnummer ABIN272279

Produktdetails anti-CDC27 Antikörper

Target: CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CDC27 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: This antibody detects endogenous levels of Cdc27 protein. (region surrounding Lys390)

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Reinheit: > 95 % pure by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: DO NOT FREEZE!

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.

Details zu CDC27

Target: CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))

Andere Bezeichnung: CDC27 (CDC27 Produkte)

Synonyme: ANAPC3 antikoerper, APC3 antikoerper, CDC27Hs antikoerper, D0S1430E antikoerper, D17S978E antikoerper, HNUC antikoerper, NUC2 antikoerper, wu:fb54g06 antikoerper, wu:fc88c12 antikoerper, AI452358 antikoerper, BC023187 antikoerper, CDC27 antikoerper, NV10210 antikoerper, cell division cycle 27 antikoerper, cell division cycle 27 S homeolog antikoerper, cell division cycle protein 27 homolog antikoerper, anaphase-promoting complex subunit Apc3 antikoerper, CDC27 antikoerper, cdc27 antikoerper, Cdc27 antikoerper, cdc27.S antikoerper, LOC100123129 antikoerper, nuc2 antikoerper

Hintergrund: Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division cycle (Cdc) genes are required at various points in the cell cycle. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6,which is involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with HSP 90. Cdc34, Cdc27 and Cdc16 function as ubiquitinconjugating enzymes. Cdc34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae Cdc34, which is essential for the G1 to S phase transition. Cdc16 and Cdc27 are components of the APC (anaphasepromoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation.Synonyms: CDC27Hs, Cell division cycle protein 27 homolog, D0S1430E, D17S978E, H-NUC

Molekulargewicht: approx. 91 kDa

Gen-ID: 996

NCBI Accession: NP_001107563

UniProt: P30260