CDC27 Antikörper (pThr244)

Produktnummer ABIN129656

Produktdetails anti-CDC27 Antikörper

Target: CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))

Bindungsspezifität: AA 239-249, pThr244

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CDC27 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktmerkmale: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 239-249 of Human CDC27.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 92 kDa in size corresponding to CDC27 by western blotting in the appropriate cell lysate or extract.  Less than 1% reactivity is observed against the non-phosphorylated form of the immunizing peptide.  This antibody is phospho specific for pT244 of CDC27.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Details zu CDC27

Target: CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))

Andere Bezeichnung: CDC27 (CDC27 Produkte)

Synonyme: ANAPC3 antikoerper, APC3 antikoerper, CDC27Hs antikoerper, D0S1430E antikoerper, D17S978E antikoerper, HNUC antikoerper, NUC2 antikoerper, wu:fb54g06 antikoerper, wu:fc88c12 antikoerper, AI452358 antikoerper, BC023187 antikoerper, CDC27 antikoerper, NV10210 antikoerper, cell division cycle 27 antikoerper, cell division cycle 27 S homeolog antikoerper, cell division cycle protein 27 homolog antikoerper, anaphase-promoting complex subunit Apc3 antikoerper, CDC27 antikoerper, cdc27 antikoerper, Cdc27 antikoerper, cdc27.S antikoerper, LOC100123129 antikoerper, nuc2 antikoerper

Hintergrund: Human CDC27 (also called Cell division cycle protein 27 homolog, CDC27Hs and H-NUC) shares strong similarity with Saccharomyces cerevisiae protein Cdc27.  This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of a cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis.
Synonyms: Cell division cycle protein 27 homolog CDC27Hs H-NUC

Gen-ID: 996, 167466175

UniProt: P30260