NSUN2 Antikörper

Produktnummer ABIN2459234

Produktdetails anti-NSUN2 Antikörper

Target: NSUN2 (NOP2/Sun Domain Family, Member 2 (NSUN2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NSUN2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Aufreinigung: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NSUN2.

Anwendungsinformationen

Applikationshinweise: NSUN2 antibody can be used for detection of NSUN2 by ELISA at 1:62500. NSUN2 antibody can be used for detection of NSUN2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Konzentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handhabung: As with any antibody avoid repeat freeze-thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: For short periods of storage (days) store at 4 °C. For longer periods of storage, store NSUN2 antibody at -20 °C.

Details zu NSUN2

Target: NSUN2 (NOP2/Sun Domain Family, Member 2 (NSUN2))

Andere Bezeichnung: NSUN2 (NSUN2 Produkte)

Synonyme: D13Wsu123e antikoerper, Misu antikoerper, MISU antikoerper, MRT5 antikoerper, SAKI antikoerper, TRM4 antikoerper, RGD1311954 antikoerper, NOL1/NOP2/Sun domain family member 2 antikoerper, NOP2/Sun RNA methyltransferase family member 2 antikoerper, NOP2/Sun RNA methyltransferase family, member 2 antikoerper, NOP2/Sun RNA methyltransferase family member 2 S homeolog antikoerper, Nsun2 antikoerper, NSUN2 antikoerper, nsun2.S antikoerper

Hintergrund: Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu (CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 is a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu (CAA).Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu (CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 encodes a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu (CAA) (Brzezicha et al., 2006 [PubMed 17071714]).

Molekulargewicht: 86 kDa

Gen-ID: 54888

NCBI Accession: NP_060225

UniProt: Q08J23