NSUN2 Antikörper (C-Term)

Produktnummer ABIN2783512

Zitiert in 1 Publikation. Der Kaninchen Polyklonal anti-NSUN2 Antikörper (ABIN2783512) detektiert spezifisch NSUN2 in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte, Rind (Kuh), Hund, Pferd, Meerschweinchen, Kaninchen und Schwein.

Kurzübersicht für NSUN2 Antikörper (C-Term) (ABIN2783512)

Target: NSUN2 (NOP2/Sun Domain Family, Member 2 (NSUN2))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Pferd, Meerschweinchen, Kaninchen, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NSUN2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-NSUN2 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: NSUN2 Antibody - C-terminal region

Sequenz: FINSRIITVS MEDVKILLTQ ENPFFRKLSS ETYSQAKDLA KGSIVLKYEP

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 93%, Rat: 100%

Produktmerkmale: This is a rabbit polyclonal antibody against NSUN2. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human NSUN2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8106631

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: prevent freeze-thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-NSUN2 Antikörper (ABIN2783512)

Blanco, Bandiera, Popis, Hussain, Lombard, Aleksic, Sajini, Tanna, Cortés-Garrido, Gkatza, Dietmann, Frye: "Stem cell function and stress response are controlled by protein synthesis." in: Nature, Vol. 534, Issue 7607, pp. 335-40, (2016) (PubMed).

Details zu NSUN2

Target: NSUN2 (NOP2/Sun Domain Family, Member 2 (NSUN2))

Andere Bezeichnung: NSUN2

Hintergrund:

Background Information: Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu(CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 is a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu(CAA).Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu(CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 encodes a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu(CAA) (Brzezicha et al., 2006 [PubMed 17071714]).[supplied by OMIM].

Gene Name: NOP2/Sun domain family, member 2

Alternative Symbols: MISU, MRT5, SAKI, TRM4

Protein Name: tRNA (cytosine(34)-C(5))-methyltransferase

Molekulargewicht: 86 kDa

Gen-ID: 54888

NCBI Accession: NP_060225

UniProt: Q08J23