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GFP Antikörper (Green Fluorescent Protein) (AA 183-191)

Details für Produkt anti-GFP Antikörper Nr. ABIN1742292, Anbieter: Anmelden zum Anzeigen
Antigen
Epitop
AA 183-191
24
18
10
6
5
3
3
2
2
2
2
1
1
1
1
1
Reaktivität
Aequorea victoria
510
5
3
2
2
2
2
1
Wirt
Maus
221
163
79
38
11
8
2
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser GFP Antikörper ist unkonjugiert
31
30
22
15
13
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Western Blotting (WB)
423
248
132
126
113
47
45
38
35
26
15
12
10
9
8
4
3
2
2
1
1
1
1
1
1
1
1
1
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Immunogen Recombinant full length GFP, glutaraldehyde fixed. Epitope QQNTPIGDG (aa 183-191)
Klon 101G4
Isotyp IgG2b
Spezifität Specific for GFP of jelly-fish.
Reinigung purified IgG. Azide was added before lyophilization.
Andere Bezeichnung GFP
Forschungsgebiet Tags/Labels
Applikationshinweise WB: 1 : 1000 up to 1 : 10 000 (AP staining)
IP: no (methanol fixated material only)
IHC: not tested yet
Kommentare

ABIN1742292 is recommended for IP and ICC.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS, 0.02% sodium azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/ml protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
Lagerung -20 °C
Informationen zur Lagerung Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Bilder des Herstellers
Western Blotting (WB) image for anti-GFP Antikörper (Green Fluorescent Protein) (AA 183-191) (ABIN1742292) dilution: 1 : 1000, sample: recombinant protein
Produkt verwendet in: Koestler, Steffen, Nemethova et al.: "Arp2/3 complex is essential for actin network treadmilling as well as for targeting of capping protein and cofilin." in: Molecular biology of the cell, Vol. 24, Issue 18, pp. 2861-75, 2013 (PubMed).

Oelkers, Vinzenz, Nemethova et al.: "Microtubules as platforms for assaying actin polymerization in vivo." in: PLoS ONE, Vol. 6, Issue 5, pp. e19931, 2011 (PubMed).