GFP Antikörper (Green Fluorescent Protein) (TRITC)

Details for Product anti-GFP Antibody No. ABIN116750, Anbieter: Anmelden zum Anzeigen
Antigen
  • green fluorescent protein
  • gfp
Reaktivität
Aequorea victoria
638
16
5
5
2
2
Wirt
Ziege
340
171
79
40
16
9
9
2
Klonalität
Polyklonal
Konjugat
Dieser GFP Antikörper ist konjugiert mit TRITC
33
31
28
14
12
12
12
10
9
9
8
8
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4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
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1
Applikation
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
536
354
201
156
145
134
96
87
73
24
15
13
13
8
8
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3
3
3
3
2
2
2
1
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Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen GST-Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria
Spezifität This antibody detects Fluorescent Protein (GFP). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Peroxidase and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum. No reaction was observed against Human, Mouse and Rat Serum Proteins.
Produktmerkmale Absorption / Emission: 550 nm / 570 nm
Molar Ratio: 2.9 moles TRITC per mole of Goat IgG
Reinigung Immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
Andere Bezeichnung GFP
Substanzklasse Tag
Hintergrund Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y, antibodies raised against full-length GFP should also detect YFP and other variants.Synonyms: GFP-Tag, Green fluorescent protein
Gen-ID 6100
UniProt P42212
Forschungsgebiet Tags/Labels
Applikationshinweise ELISA (Sandwich or Capture, ELISA for direct binding recognizes wild type, recombinantand enhanced forms of GFP, for sandwich ELISA titrate GFP in solution using either form ofthe antibody as the capture or detection antibodies, detection antibody is typically
Beschränkungen Nur für Forschungszwecke einsetzbar
Rekonstitution Restore with 1.0 mL of deionized water (or equivalent).
Konzentration 1.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0 as a buffer, 10 mg/mL Bovine Serum Albumin (BSA) IgG and (protease free) as stabilizer, 0.01 % (w/v) Sodium azide as preservative
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing. Protect from light.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Produkt verwendet in: Niv, Keiner, Krishna, Witte, Lie, Redecker: "Aberrant neurogenesis after stroke: a retroviral cell labeling study." in: Stroke; a journal of cerebral circulation, Vol. 43, Issue 9, pp. 2468-75, 2012 (PubMed).

Suzuki, Mogami, Ihara, Urano: "Unique secretory dynamics of tissue plasminogen activator and its modulation by plasminogen activator inhibitor-1 in vascular endothelial cells." in: Blood, Vol. 113, Issue 2, pp. 470-8, 2009 (PubMed).

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