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CD44 Antikörper (CD44) (FITC)

Details für Produkt anti-CD44 Antikörper Nr. ABIN114365, Anbieter: Anmelden zum Anzeigen
Antigen
  • AU023126
  • AW121933
  • AW146109
  • HERMES
  • Ly-24
  • Pgp-1
  • CD44A
  • METAA
  • RHAMM
  • CDW44
  • CSPG8
  • ECMR-III
  • HCELL
  • HUTCH-I
  • IN
  • LHR
  • MC56
  • MDU2
  • MDU3
  • MIC4
  • Pgp1
  • CD44 antigen
  • CD44 antigen-like
  • Cd44 molecule
  • CD44 molecule (Indian blood group)
  • syndecan 4
  • Cd44
  • LOC100525150
  • CD44
  • SDC4
Alternativen
anti-Ratte (Rattus) CD44 Antikörper für Flow Cytometry
Reaktivität
Ratte (Rattus)
1192
451
210
108
60
60
58
50
46
45
38
36
32
29
28
12
6
6
3
3
2
2
2
1
1
1
1
1
1
Wirt
Maus
1027
289
221
17
2
2
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser CD44 Antikörper ist konjugiert mit FITC
200
122
98
42
31
30
29
25
23
23
20
20
20
20
20
17
12
11
9
8
8
8
7
7
7
5
4
4
4
4
4
4
4
4
2
2
2
2
2
2
1
Applikation
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
1106
632
505
326
302
294
276
272
199
54
21
20
18
17
16
14
13
8
7
7
7
7
6
6
5
5
5
2
2
1
1
1
1
1
Optionen
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Immunogen T cell blasts.
Klon OX-49
Isotyp IgG2a
Spezifität This anti-Rat CD44 monoclonal antibody recognizes an epitope on both standard CD44 and its splice variant.
Kreuzreaktivität (Details) Species reactivity (tested):Rat
Reinigung Protein G affinity chromatography
Andere Bezeichnung CD44 (CD44 Antibody Abstract)
Hintergrund This antigen is expressed on most leukocytes (except a sub population of B cells) and increases upon activation. This antibody binds extracellularly to the standard (S) form on rat leukocytes, but it is not known if they bind to the N-terminal region. It has also been reported that the antibody may bind to melanoma cell lines that express CD44V (splice variant form). CD44 is expressed on most leukocytes except a sub population of B cells. Its expression is increased on T and B blasts.Synonyms: CDw44, ECMR-III, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, LHR, MDU2, MDU3, MIC4, PGP-1, Phagocytic glycoprotein 1
Gen-ID 10116
UniProt P26051
Forschungsgebiet Stem Cells, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Cancer, Cell Cycle
Pathways Glycosaminoglycan Metabolic Process
Applikationshinweise Suitable for Flow cytometry (See protocol) and Immunohistochemistry on frozen andparaffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protokoll FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Rat cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 1. 0-0. 5 µg* of ABIN114364 or ABIN114364X. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes are protected from light since most fluorochromes arelight sensitive). 7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results-Tissue Distribution: Rat Strain: WistarCell Concentration: 1 x 10e6 cells per testsAntibody Concentration Used: 1. 0 µg/10e6 cellsIsotypic Control: FITC Mouse IgG2a. Cell Source Percentage of cells stained above control: Thymus: 99. 6%Spleen: 75. 9%Lymph Node: 96. 8%N. B. Appropriate control samples should always be included in any labelling studies. * For optimal results in various applications, it is recommended that each investigatordetermine dilutions appropriate for individual use.
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 0.1 mg/mL
Buffer PBS with 0.02 % sodium azide as preservative and EIA grade BSA as a stabilizer
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Bilder des Herstellers
Flow Cytometry (FACS) image for anti-CD44 Antikörper (CD44)  (FITC) (ABIN114365) Mouse anti CD44 (HCAM) OX-49
Allgemeine Veröffentlichungen Arch, Wirth, Hofmann, Ponta, Matzku, Herrlich, Zöller: "Participation in normal immune responses of a metastasis-inducing splice variant of CD44." in: Science (New York, N.Y.), Vol. 257, Issue 5070, pp. 682-5, 1992 (PubMed).