Maus Heat Shock 60kDa Protein 1 (Chaperonin) ELISA Kit

Recommended Heat Shock 60kDa Protein 1 (Chaperonin) Product (geliefert von: Anmelden zum Anzeigen )

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Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) ELISA Kits
  • 12
  • BP5
  • CG12101
  • Cpn60
  • Dmel\\CG12101
  • Dmhsp60
  • G62
  • HSP60
  • HSP60A
  • Hsp60A
  • IEF16
  • Mmp-P1
  • SSP 7506
  • cpn60
  • d-hsp60
  • hsp60
  • hsp60A
  • l(1)10Ac
  • l(1)BP5
  • l(1)G8
  • l(1)HM21
  • l(1)L12
  • l(1)dp025
  • HSPD1
  • hld4
  • groel
  • hsp65
  • spg13
  • chaperonin
  • CPN60
  • MIF4
  • MNA2
  • mopA
  • groL
  • crpA
  • cb863
  • fa04a05
  • fb22d10
  • fi27b05
  • id:ibd2197
  • sb:cb144
  • wu:fa04a05
  • wu:fb22d10
  • wu:fi04a12
  • wu:fi27b05
  • GROEL
  • HLD4
  • HSP-60
  • HSP65
  • HuCHA60
  • SPG13
  • 60kDa
  • Hsp60
  • Hspd1-30p
  • Heat shock protein 60
  • heat shock 60kDa protein 1 (chaperonin)
  • chaperone ATPase HSP60
  • molecular chaperone GroEL
  • chaperonin GroEL
  • mitochondrial chaperonin
  • heat shock protein 60
  • heat shock 60kD protein 1 (chaperonin)
  • heat shock protein 1 (chaperonin)
  • Hsp60
  • HSPD1
  • hspd1
  • HSP60
  • groEL
  • hsp60
  • groEl
  • Hspd1
Reaktivität
Maus
Alternativen
Kits mit alternativen Reaktivitäten:
20
14
13
7
5
4
3
3
3
3
2
1
1
1
Methodentyp
Sandwich ELISA
Detektionsbereich
0.234-15 ng/mL
Untere Nachweisgrenze
0.234 ng/mL
Applikation
ELISA
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN1570417
$ 852.24
Zzgl. Versandkosten $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Hersteller References Details
10.090268 ABIN415418 Sandwich ELISA Plasma, Cell Lysate, Cell Culture Supernatant, Serum, Tissue Homogenate, Biological Fluids 1.56-100 ng/mL 1.56 ng/mL Anmelden zum Anzeigen
10.090268 ABIN367883 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate 0.9-60 ng/mL 0.9 ng/mL Anmelden zum Anzeigen
10.090268 ABIN1115349 Sandwich ELISA Serum, Plasma, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Anmelden zum Anzeigen
1 ABIN627935 Competition ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 0.5-10 ng/mL 0.5 ng/mL Anmelden zum Anzeigen
1 ABIN4883232 Sandwich ELISA Plasma, Cell Culture Supernatant, Serum Anmelden zum Anzeigen
1 ABIN5654954 Sandwich ELISA Biological Fluids, Cell Lysate, Plasma, Serum, Tissue Homogenate 0.234 ng/mL - 15 ng/mL 0.234 ng/mL Anmelden zum Anzeigen
1 ABIN5592174 Sandwich ELISA Biological Fluids, Plasma, Serum, Tissue Homogenate 0.156-10 ng/mL 0.156 ng/mL Anmelden zum Anzeigen
1 ABIN992541 Competition ELISA Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate 25-500 ng/mL 25 ng/mL Anmelden zum Anzeigen
1 ABIN5520799 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 0.156-10 ng/mL 0.156 ng/mL Anmelden zum Anzeigen
1 ABIN2534246 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Biological Fluids 0.234-15 ng/mL 0.234 ng/mL Anmelden zum Anzeigen
1 ABIN2948159 0.156-10 ng/mL 0.156 ng/mL Anmelden zum Anzeigen
1 ABIN455305 0.156-10 ng/mL 0.156 ng/mL Anmelden zum Anzeigen
1 ABIN5069100 Sandwich ELISA Plasma, Serum, Tissue Homogenate, Biological Fluids Anmelden zum Anzeigen

General

Antigen Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) ELISA Kits
Reaktivität Maus
Kits mit alternativen Reaktivitäten:
(20), (14), (13), (7), (5), (4), (3), (3), (3), (3), (2), (1), (1), (1)
Methodentyp Sandwich ELISA
Detektionsbereich 0.234-15 ng/mL
Untere Nachweisgrenze 0.234 ng/mL
Applikation ELISA
Hersteller Anmelden zum Anzeigen

Produktdetails

Antigendetails Anwendungsinformationen Handhabung Bilder
Verwendungszweck The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of HSPD1 in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Proben Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids
Nachweismethode Colorimetric
Analytische Methode Quantitative
Spezifität This assay has high sensitivity and excellent specificity for detection of this index.
Kreuzreaktivität (Details) No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
Sensitivität 0.11 ng/mL
Bestandteile
  • Pre-coated, ready to use 96-well strip plate
  • Standard (freeze dried)
  • Standard Diluent
  • Detection Reagent A
  • Detection Reagent B
  • Assay Diluent A
  • Assay Diluent B
  • TMB
  • Stop Solution
  • Wash Buffer (30X)
  • Plate sealer for 96 wells
  • Instruction manual
Benötigtes Material
  1. Microplate reader with 450 ± 10nm filter.
  2. Precision single or multi-channel pipettes and disposable tips.
  3. Eppendorf Tubes for diluting samples.
  4. Deionized or distilled water.
  5. Absorbent paper for blotting the microtiter plate.
  6. Container for Wash Solution.

Antigendetails

Produktdetails Anwendungsinformationen Handhabung Bilder zurück nach oben
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Andere Bezeichnung Heat Shock 60kD Protein 1, Chaperonin (HSPD1) (HSPD1 ELISA Kit Abstract)
Hintergrund Synonyms: 60 kDa chaperonin,60 kDa heat shock protein, mitochondrial,Chaperonin 60,CPN60,Heat shock protein 60,Hsp60,HSP-60,HSP-65,Mitochondrial matrix protein P1
Gen-ID 15510
UniProt P63038
Pathways Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity

Anwendungsinformationen

Produktdetails Antigendetails Handhabung Bilder zurück nach oben
Probenmenge 100 μL
Testdauer 1 - 4.5 h
Plattentyp Pre-coated
Protokoll 1. Prepare all reagents, samples and standards
2. Add 100 µL standard or sample to each well. Incubate 2 hours at 37 °C
3. Aspirate and add 100 µL prepared Detection Reagent A. Incubate 1 hour at 37 °C
4. Aspirate and wash 3 times
5. Add 100 µL prepared Detection Reagent B. Incubate 30 minutes at 37 °C
6. Aspirate and wash 5 times
7. Add 90 µL Substrate Solution. Incubate 15-25 minutes at 37 °C
8. Add 50 µL Stop Solution. Read at 450nm immediately.
Testdurchführung The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Testpräzision
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
  • CV(%) = SD/meanX100
  • Intra-assay: CV&lt10%
  • Inter-assay: CV&lt12%
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails Antigendetails Anwendungsinformationen Bilder zurück nach oben
Vorsichtsmaßnahmen The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Handhabung The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
Haltbarkeit 12 months

Bilder

Produktdetails Antigendetails Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
ELISA image for Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) ELISA Kit (ABIN1570417) Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) ELISA Kit