anti-Ratte (Rattus) Heat Shock 60kDa Protein 1 (Chaperonin) Antikörper für BioImaging

Recommended Heat Shock 60kDa Protein 1 (Chaperonin) Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) Antikörper
  • 12
  • BP5
  • CG12101
  • Cpn60
  • Dmel\\CG12101
  • Dmhsp60
  • G62
  • HSP60
  • HSP60A
  • Hsp60A
  • IEF16
  • Mmp-P1
  • SSP 7506
  • cpn60
  • d-hsp60
  • hsp60
  • hsp60A
  • l(1)10Ac
  • l(1)BP5
  • l(1)G8
  • l(1)HM21
  • l(1)L12
  • l(1)dp025
  • HSPD1
  • hld4
  • groel
  • hsp65
  • spg13
  • chaperonin
  • CPN60
  • MIF4
  • MNA2
  • mopA
  • groL
  • crpA
  • cb863
  • fa04a05
  • fb22d10
  • fi27b05
  • id:ibd2197
  • sb:cb144
  • wu:fa04a05
  • wu:fb22d10
  • wu:fi04a12
  • wu:fi27b05
  • GROEL
  • HLD4
  • HSP-60
  • HSP65
  • HuCHA60
  • SPG13
  • 60kDa
  • Hsp60
  • Hspd1-30p
  • Heat shock protein 60
  • heat shock 60kDa protein 1 (chaperonin)
  • chaperone ATPase HSP60
  • molecular chaperone GroEL
  • chaperonin GroEL
  • mitochondrial chaperonin
  • heat shock protein 60
  • heat shock 60kD protein 1 (chaperonin)
  • heat shock protein 1 (chaperonin)
  • Hsp60
  • HSPD1
  • hspd1
  • HSP60
  • groEL
  • hsp60
  • groEl
  • Hspd1
Reaktivität
Human, Maus, Ratte (Rattus)
652
435
417
271
245
227
220
205
176
167
139
117
116
96
94
67
42
41
36
36
22
21
21
21
19
19
18
17
17
4
4
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Wirt
Maus
421
280
9
Klonalität
Monoklonal
Konjugat
Unkonjugiert
26
19
18
17
14
12
11
11
11
11
11
10
10
9
9
9
9
9
8
8
8
8
8
8
8
8
8
8
4
4
4
4
3
Applikation
BioImaging (BI), Western Blotting (WB)
622
289
288
278
266
262
198
187
84
13
12
10
8
7
5
2
1
1
1
Optionen
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Bilder

Produktnummer ABIN968590
Preis und Verfügbarkeit auf Anfrage.
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN968591 BI WB Mouse IgG1 Anmelden zum Anzeigen 24-HSP60 5

General

Antigen Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) Antikörper
Reaktivität Human, Maus, Ratte (Rattus)
(652), (435), (417), (271), (245), (227), (220), (205), (176), (167), (139), (117), (116), (96), (94), (67), (42), (41), (36), (36), (22), (21), (21), (21), (19), (19), (18), (17), (17), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Wirt Maus
(421), (280), (9)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Unkonjugiert
(26), (19), (18), (17), (14), (12), (11), (11), (11), (11), (11), (10), (10), (9), (9), (9), (9), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (4), (4), (4), (4), (3)
Applikation BioImaging (BI), Western Blotting (WB)
(622), (289), (288), (278), (266), (262), (198), (187), (84), (13), (12), (10), (8), (7), (5), (2), (1), (1), (1)
Pubmed 5 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails

Antigendetails Anwendungsinformationen Handhabung Referenzen Bilder
Kreuzreaktivität Maus, Ratte (Rattus)
Produktmerkmale 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
6. Triton is a trademark of the Dow Chemical Company.
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human Hsp60 Recombinant Protein
Klon 24-HSP60
Isotyp IgG1

Antigendetails

Produktdetails Anwendungsinformationen Handhabung Referenzen Bilder zurück nach oben
Antigen
Andere Bezeichnung Hsp60 (HSPD1 Antibody Abstract)
Hintergrund Heat shock proteins (Hsp) are a set of highly conserved proteins that include constitutively expressed (Hsp60, Hsp70, and Hsp90) and stress-induced (Hsp27 and Hsp72) proteins. Hsp60 is localized to the mitochondria, where it promotes mitochondrial protein folding and facilitates proteolytic degradation of misfolded or denatured proteins. It binds Hsp10, which regulates the substrate binding and ATPase activity of Hsp60. In HeLa and Jurkat mitochondria, Hsp60 associates with caspase-3 to form a complex that dissociates and releases from the mitochondria during apoptosis. In addition, Hsp60 accelerates the maturation of procaspase-3 through its ATP-dependent foldase activity. In addition to its role in protein folding, Hsp60 has also been implicated in immune function. In macrophages, its binding to the toll-like receptor-4 complex induces production of TNFalpha and nitric oxide and stimulation of a proinflammatory response. Thus, the protein folding function of Hsp60 is involved in mitochondrial protein folding in both normal and apoptotic cells, while release of Hsp60 during necrosis is thought to stimulate a proinflammatory response.
Molekulargewicht 60 kDa
Forschungsgebiet Signaling, Heat Shock Proteins
Pathways Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity

Anwendungsinformationen

Produktdetails Antigendetails Handhabung Referenzen Bilder zurück nach oben
Applikationshinweise Bioimaging
1. Seed the cells in appropriate culture medium at ~10,000 cells per well in an 96-well Imaging Plate and culture overnight.
2. Remove the culture medium from the wells, and fix the cells by adding 100 myl of Fixation Buffer to each well. Incubate for 10 minutes at room temperature (RT).
3. Remove the fixative from the wells, and permeabilize the cells using either 90% methanol, or Triton™ X-100: a. Add 100 myl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. OR b. Add 100 myl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT.
4. Remove the permeabilization buffer, and wash the wells twice with 100 myl of 1× PBS.
5. Remove the PBS, and block the cells by adding 100 myl of to each well. Incubate for 30 minutes at RT.
6. Remove the blocking buffer and add 50 myl of the optimally titrated primary antibody (diluted in Stain Buffer) to each well, and incubate for 1 hour at RT.
7. Remove the primary antibody, and wash the wells three times with 100 myl of 1× PBS.
8. Remove the PBS, and add the second step reagent at its optimally titrated concentration in 50 myl to each well, and incubate in the dark for 1 hour at RT.
9. Remove the second step reagent, and wash the wells three times with 100 myl of 1× PBS.
10. Remove the PBS, and counter-stain the nuclei by adding 200 myl per well of 2 myg/ml Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
11. View and analyze the cells on an appropriate imaging instrument.
Kommentare

Related Products: ABIN967389, ABIN968537

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails Antigendetails Anwendungsinformationen Referenzen Bilder zurück nach oben
Format Liquid
Konzentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store undiluted at -20°C.

Referenzen

Produktdetails Antigendetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Ohashi, Burkart, Flohé, Kolb: "Cutting edge: heat shock protein 60 is a putative endogenous ligand of the toll-like receptor-4 complex." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 2, pp. 558-61, 2000

Xanthoudakis, Roy, Rasper, Hennessey, Aubin, Cassady, Tawa, Ruel, Rosen, Nicholson: "Hsp60 accelerates the maturation of pro-caspase-3 by upstream activator proteases during apoptosis." in: The EMBO journal, Vol. 18, Issue 8, pp. 2049-56, 1999

Venner, Singh, Gupta: "Nucleotide sequences and novel structural features of human and Chinese hamster hsp60 (chaperonin) gene families." in: DNA and cell biology, Vol. 9, Issue 8, pp. 545-52, 1991

Gutiérrez, Rojas, Lomonte, Gené, Chaves, Alvarado, Rojas: "Standardization of assays for testing the neutralizing ability of antivenoms." in: Toxicon : official journal of the International Society on Toxinology, Vol. 28, Issue 10, pp. 1127-9; author reply 1129-32, 1991

Allgemeine Veröffentlichungen

Samali, Cai, Zhivotovsky, Jones, Orrenius: "Presence of a pre-apoptotic complex of pro-caspase-3, Hsp60 and Hsp10 in the mitochondrial fraction of jurkat cells." in: The EMBO journal, Vol. 18, Issue 8, pp. 2040-8, 1999

Bilder

Produktdetails Antigendetails Anwendungsinformationen Handhabung Referenzen zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) antibody (ABIN968590) Western blot analysis of Hsp60 on a Jurkat lysate (ABIN968537). Lane 1: 1:5000, lane ...
Immunofluorescence (IF) image for anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) antibody (ABIN968590) Immunofluorescent staining of A549 (ATCC CCL-185) cells. Cells were seeded in a 96 we...