anti-Plasmodium falciparum Heat Shock 60kDa Protein 1 (Chaperonin) Antikörper für Western Blotting

Recommended Heat Shock 60kDa Protein 1 (Chaperonin) Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) Antikörper
  • 12
  • BP5
  • CG12101
  • Cpn60
  • Dmel\\CG12101
  • Dmhsp60
  • G62
  • HSP60
  • HSP60A
  • Hsp60A
  • IEF16
  • Mmp-P1
  • SSP 7506
  • cpn60
  • d-hsp60
  • hsp60
  • hsp60A
  • l(1)10Ac
  • l(1)BP5
  • l(1)G8
  • l(1)HM21
  • l(1)L12
  • l(1)dp025
  • HSPD1
  • hld4
  • groel
  • hsp65
  • spg13
  • chaperonin
  • CPN60
  • MIF4
  • MNA2
  • mopA
  • groL
  • crpA
  • cb863
  • fa04a05
  • fb22d10
  • fi27b05
  • id:ibd2197
  • sb:cb144
  • wu:fa04a05
  • wu:fb22d10
  • wu:fi04a12
  • wu:fi27b05
  • GROEL
  • HLD4
  • HSP-60
  • HSP65
  • HuCHA60
  • SPG13
  • 60kDa
  • Hsp60
  • Hspd1-30p
  • Heat shock protein 60
  • heat shock 60kDa protein 1 (chaperonin)
  • chaperone ATPase HSP60
  • molecular chaperone GroEL
  • chaperonin GroEL
  • mitochondrial chaperonin
  • heat shock protein 60
  • heat shock 60kD protein 1 (chaperonin)
  • heat shock protein 1 (chaperonin)
  • Hsp60
  • HSPD1
  • hspd1
  • HSP60
  • groEL
  • hsp60
  • groEl
  • Hspd1
Reaktivität
Bakterien, Plasmodium falciparum
653
436
418
271
245
227
220
205
176
167
139
117
116
96
94
66
42
41
36
35
22
21
21
21
19
19
18
17
17
4
4
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Wirt
Kaninchen
422
279
9
Klonalität
Polyklonal
Konjugat
Atto 390
26
19
18
17
14
12
11
11
11
11
11
10
10
9
9
9
9
9
8
8
8
8
8
8
8
8
8
7
4
4
4
4
3
Applikation
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
622
289
288
278
265
262
198
186
84
13
12
10
8
7
5
2
2
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Request

Produkt kostenlos erhalten?

Für Ihre Validierungsdaten erstatten wir Ihnen den vollen Kaufpreis. Ich möchte dieses Produkt validieren

Mehr erfahren

Bilder

Produktnummer ABIN2484788
$ 436.70
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
3.1474795 ABIN2484790 ICC IF WB Atto 565 Rabbit Anmelden zum Anzeigen Polyclonal
3.1474795 ABIN2484791 ICC IF WB Atto 594 Rabbit Anmelden zum Anzeigen Polyclonal
3.1474795 ABIN2484792 ICC IF WB Atto 633 Rabbit Anmelden zum Anzeigen Polyclonal
3.1474795 ABIN2484794 ICC IF WB Atto 680 Rabbit Anmelden zum Anzeigen Polyclonal
3.1474795 ABIN2484793 ICC IF WB Atto 655 Rabbit Anmelden zum Anzeigen Polyclonal
3.1474795 ABIN2484795 ICC IF WB Atto 700 Rabbit Anmelden zum Anzeigen Polyclonal
3.1474795 ABIN2484796 ICC IF WB Alkaline Phosphatase (AP) Rabbit Anmelden zum Anzeigen Polyclonal
3.1474795 ABIN2484797 ICC IF WB APC Rabbit Anmelden zum Anzeigen Polyclonal
3.1474795 ABIN2484798 ICC IF WB Biotin Rabbit Anmelden zum Anzeigen Polyclonal
3.1474795 ABIN2484799 ICC IF WB FITC Rabbit Anmelden zum Anzeigen Polyclonal
3.1474795 ABIN2484800 ICC IF WB HRP Rabbit Anmelden zum Anzeigen Polyclonal
3.1474795 ABIN2484804 ICC IF WB Streptavidin Rabbit Anmelden zum Anzeigen Polyclonal
3.1474795 ABIN2484803 ICC IF WB PE Rabbit Anmelden zum Anzeigen Polyclonal
3.1474795 ABIN2484789 ICC IF WB Atto 488 Rabbit Anmelden zum Anzeigen Polyclonal
3.1474795 ABIN2484801 ICC IF WB PE-Atto 594 Rabbit Anmelden zum Anzeigen Polyclonal
3.1474795 ABIN2484802 ICC IF WB PerCP Rabbit Anmelden zum Anzeigen Polyclonal
1 ABIN361856 ICC IF WB Rabbit Anmelden zum Anzeigen Polyclonal 10
1 ABIN467065 IF WB Rabbit Anmelden zum Anzeigen Polyclonal
1 ABIN4221876 ICC IF WB Rabbit Anmelden zum Anzeigen Polyclonal
1 ABIN1968055 IF WB Atto 680 Rabbit Anmelden zum Anzeigen Polyclonal

General

Antigen Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) Antikörper
Reaktivität Bakterien, Plasmodium falciparum
(653), (436), (418), (271), (245), (227), (220), (205), (176), (167), (139), (117), (116), (96), (94), (66), (42), (41), (36), (35), (22), (21), (21), (21), (19), (19), (18), (17), (17), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Wirt Kaninchen
(422), (279), (9)
Klonalität
Polyklonal
Konjugat Atto 390
(26), (19), (18), (17), (14), (12), (11), (11), (11), (11), (11), (10), (10), (9), (9), (9), (9), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (7), (4), (4), (4), (4), (3)
Applikation Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(622), (289), (288), (278), (265), (262), (198), (186), (84), (13), (12), (10), (8), (7), (5), (2), (2), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails

Antigendetails Anwendungsinformationen Handhabung Bilder
Spezifität Detects ~ 60 kDa. Cross-reacts with E.coli HSP60, GroEl.
Reinigung Protein A Purified
Immunogen Recombinant full length PfHSP60

Antigendetails

Produktdetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung HSP60 (HSPD1 Antibody Abstract)
Hintergrund In both prokaryotic and eukaryotic cells, the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones. Members of the HSP60 family of heat shock proteins are some of the best characterized chaperones. HSP60, also known as Cpn60 or GroEl, is an abundant protein synthesized constitutively in the cell that is induced to a higher concentration after brief cell shock. It is present in many species and exhibits a remarkable sequence homology among various counterparts in bacteria, plants, and mammals with more than half of the residues identical between bacterial and mammalian HSP60 (1-3). Whereas mammalian HSP60 is localized within the mitochondria, plant HSP60, or otherwise known as Rubisco-binding protein, is located in plant chloroplasts. It has been indicated that these proteins carry out a very important biological function due to the fact that HSP60 is present in so many different species. The common characteristics of the HSP60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo-oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of Mg2+ and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures (4). These similarities are supported by recent studies where the single-ring human mitochondrial homolog, HSP60 with its co-chaperonin, HSP10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of HSP60-HSP10 was able to carry out all essential in vivo functions of GroEL and its co-chaperonin, GroES (5). Another important function of HSP60 and HSP10 is their protective functions against infection and cellular stress. HSP60 has however been linked to a number of autoimmune diseases, as well as Alzheimer's, coronary artery diseases, MS, and diabetes (6-9).
NCBI Accession XM_001347402
UniProt P34940
Forschungsgebiet Heat Shock Proteins
Pathways Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity

Anwendungsinformationen

Produktdetails Antigendetails Handhabung Bilder zurück nach oben
Applikationshinweise
  • WB (1:2000)
  • optimal dilutions for assays should be determined by the user.
Kommentare

0.9 μg/ml of SPC-185 was sufficient for detection of PfHSP60 in 20 μg of P. falciparum lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails Antigendetails Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration 1.83 mg/mL
Buffer PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C

Bilder

Produktdetails Antigendetails Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) antibody (Atto 390) (ABIN2484788) Western blot analysis of Parasite Lysates showing detection of HSP60 protein using Ra...