anti-Schwein Heat Shock 60kDa Protein 1 (Chaperonin) Antikörper für Immunohistochemistry

Recommended Heat Shock 60kDa Protein 1 (Chaperonin) Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) Antikörper
  • 12
  • BP5
  • CG12101
  • Cpn60
  • Dmel\\CG12101
  • Dmhsp60
  • G62
  • HSP60
  • HSP60A
  • Hsp60A
  • IEF16
  • Mmp-P1
  • SSP 7506
  • cpn60
  • d-hsp60
  • hsp60
  • hsp60A
  • l(1)10Ac
  • l(1)BP5
  • l(1)G8
  • l(1)HM21
  • l(1)L12
  • l(1)dp025
  • HSPD1
  • hld4
  • groel
  • hsp65
  • spg13
  • chaperonin
  • CPN60
  • MIF4
  • MNA2
  • mopA
  • groL
  • crpA
  • cb863
  • fa04a05
  • fb22d10
  • fi27b05
  • id:ibd2197
  • sb:cb144
  • wu:fa04a05
  • wu:fb22d10
  • wu:fi04a12
  • wu:fi27b05
  • GROEL
  • HLD4
  • HSP-60
  • HSP65
  • HuCHA60
  • SPG13
  • 60kDa
  • Hsp60
  • Hspd1-30p
  • Heat shock protein 60
  • heat shock 60kDa protein 1 (chaperonin)
  • chaperone ATPase HSP60
  • molecular chaperone GroEL
  • chaperonin GroEL
  • mitochondrial chaperonin
  • heat shock protein 60
  • heat shock 60kD protein 1 (chaperonin)
  • heat shock protein 1 (chaperonin)
  • Hsp60
  • HSPD1
  • hspd1
  • HSP60
  • groEL
  • hsp60
  • groEl
  • Hspd1
Reaktivität
Huhn, Rind (Kuh), Hund, Fruchtfliege (Drosophila melanogaster), Meerschweinchen, Hamster, Human, Affe, Maus, Schwein, Pflanzen, Kaninchen, Ratte (Rattus), Schaf, Xenopus laevis
652
435
417
270
244
226
219
204
175
166
138
117
115
95
93
67
41
41
36
36
22
21
21
21
19
19
18
17
17
4
4
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Wirt
Maus
421
280
9
Klonalität
Monoklonal
Konjugat
PE-Atto 594
26
19
18
17
14
12
11
11
11
11
11
10
10
9
9
9
9
9
8
8
8
8
8
8
8
8
8
8
4
4
4
3
3
Applikation
Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
622
288
287
277
266
262
197
187
84
13
12
10
8
7
5
2
2
1
1
Optionen
Hersteller
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Bilder

Produktnummer ABIN2481441
$ 423.50
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
8.866798 ABIN2481444 FACS IP IHC ELISA WB Streptavidin Mouse IgG1 Anmelden zum Anzeigen LK1 2
8.866798 ABIN2481440 FACS IP IHC ELISA WB HRP Mouse IgG1 Anmelden zum Anzeigen LK1 2
8.866798 ABIN2481431 FACS IP IHC ELISA WB Atto 594 Mouse IgG1 Anmelden zum Anzeigen LK1 2
8.866798 ABIN2481435 FACS IP IHC ELISA WB Atto 700 Mouse IgG1 Anmelden zum Anzeigen LK1 2
8.866798 ABIN2481428 FACS IP IHC ELISA WB Atto 390 Mouse IgG1 Anmelden zum Anzeigen LK1 2
8.866798 ABIN2481429 FACS IP IHC ELISA WB Atto 488 Mouse IgG1 Anmelden zum Anzeigen LK1 2
8.866798 ABIN2481432 FACS IP IHC ELISA WB Atto 633 Mouse IgG1 Anmelden zum Anzeigen LK1 2
8.866798 ABIN2481433 FACS IP IHC ELISA WB Atto 655 Mouse IgG1 Anmelden zum Anzeigen LK1 2
8.866798 ABIN2481434 FACS IP IHC ELISA WB Atto 680 Mouse IgG1 Anmelden zum Anzeigen LK1 2
8.866798 ABIN2481438 FACS IP IHC ELISA WB Biotin Mouse IgG1 Anmelden zum Anzeigen LK1 2
8.866798 ABIN2481439 FACS IP IHC ELISA WB FITC Mouse IgG1 Anmelden zum Anzeigen LK1 2
8.866798 ABIN2481436 FACS IP IHC ELISA WB Alkaline Phosphatase (AP) Mouse IgG1 Anmelden zum Anzeigen LK1 2
8.866798 ABIN2481430 FACS IP IHC ELISA WB Atto 565 Mouse IgG1 Anmelden zum Anzeigen LK1 2
8.866798 ABIN2481437 FACS IP IHC ELISA WB APC Mouse IgG1 Anmelden zum Anzeigen LK1 2
8.866798 ABIN2481442 FACS IP IHC ELISA WB PerCP Mouse IgG1 Anmelden zum Anzeigen LK1 2
8.866798 ABIN2481443 FACS IP IHC ELISA WB PE Mouse IgG1 Anmelden zum Anzeigen LK1 2
8.866798 ABIN2486555 FACS IHC WB Atto 565 Mouse IgG1 Anmelden zum Anzeigen LK2 2
8.866798 ABIN2486559 FACS IHC WB Atto 680 Mouse IgG1 Anmelden zum Anzeigen LK2 2
8.866798 ABIN2486564 FACS IHC WB FITC Mouse IgG1 Anmelden zum Anzeigen LK2 2
8.866798 ABIN2486566 FACS IHC WB PE-Atto 594 Mouse IgG1 Anmelden zum Anzeigen LK2 2

General

Antigen Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) Antikörper
Reaktivität Huhn, Rind (Kuh), Hund, Fruchtfliege (Drosophila melanogaster), Meerschweinchen, Hamster, Human, Affe, Maus, Schwein, Pflanzen, Kaninchen, Ratte (Rattus), Schaf, Xenopus laevis
(652), (435), (417), (270), (244), (226), (219), (204), (175), (166), (138), (117), (115), (95), (93), (67), (41), (41), (36), (36), (22), (21), (21), (21), (19), (19), (18), (17), (17), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Wirt Maus
(421), (280), (9)
Klonalität (Klon)
Monoklonal   ( )
Konjugat PE-Atto 594
(26), (19), (18), (17), (14), (12), (11), (11), (11), (11), (11), (10), (10), (9), (9), (9), (9), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (4), (4), (4), (3), (3)
Applikation Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(622), (288), (287), (277), (266), (262), (197), (187), (84), (13), (12), (10), (8), (7), (5), (2), (2), (1), (1)
Pubmed 2 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails

Antigendetails Anwendungsinformationen Handhabung Referenzen Bilder
Spezifität Detects ~60 kDa.
Reinigung Protein G Purified
Immunogen Recombinant human HSP60
Klon LK1
Isotyp IgG1

Antigendetails

Produktdetails Anwendungsinformationen Handhabung Referenzen Bilder zurück nach oben
Antigen
Andere Bezeichnung HSP60 (HSPD1 Antibody Abstract)
Hintergrund In both prokaryotic and eukaryotic cells, the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones. Members of the HSP60 family of heat shock proteins are some of the best characterized chaperones. HSP60, also known as Cpn60 or GroEl, is an abundant protein synthesized constitutively in the cell that is induced to a higher concentration after brief cell shock. It is present in many species and exhibits a remarkable sequence homology among various counterparts in bacteria, plants, and mammals with more than half of the residues identical between bacterial and mammalian HSP60 (1-3). Whereas mammalian HSP60 is localized within the mitochondria, plant HSP60, or otherwise known as Rubisco-binding protein, is located in plant chloroplasts. It has been indicated that these proteins carry out a very important biological function due to the fact that HSP60 is present in so many different species. The common characteristics of the HSP60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo-oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of Mg2+ and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures (4). These similarities are supported by recent studies where the single-ring human mitochondrial homolog, HSP60 with its co-chaperonin, HSP10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of HSP60-HSP10 was able to carry out all essential in vivo functions of GroEL and its co-chaperonin, GroES (5). Another important function of HSP60 and HSP10 is their protective functions against infection and cellular stress. HSP60 has however been linked to a number of autoimmune diseases, as well as Alzheimer's, coronary artery diseases, MS, and diabetes (6-9).
Gen-ID 3329
NCBI Accession NP_002147
UniProt P10809
Forschungsgebiet Heat Shock Proteins
Pathways Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity

Anwendungsinformationen

Produktdetails Antigendetails Handhabung Referenzen Bilder zurück nach oben
Applikationshinweise
  • WB (1:20000)
  • IHC (1:100)
  • ICC/IF (1:100)
  • IP (1:200)
  • optimal dilutions for assays should be determined by the user.
Kommentare

0.05 μg/ml of SMC-110 was sufficient for detection of HSP60 in 20 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using goat anti-mouse IgG as the secondary antibody.

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails Antigendetails Anwendungsinformationen Referenzen Bilder zurück nach oben
Format Liquid
Konzentration 1 mg/mL
Buffer PBS, 50 % glycerol, 0.09 % sodium azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C

Referenzen

Produktdetails Antigendetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Allgemeine Veröffentlichungen

Bukau, Horwich: "The Hsp70 and Hsp60 chaperone machines." in: Cell, Vol. 92, Issue 3, pp. 351-66, 1998

Hartl: "Molecular chaperones in cellular protein folding." in: Nature, Vol. 381, Issue 6583, pp. 571-9, 1996

Bilder

Produktdetails Antigendetails Anwendungsinformationen Handhabung Referenzen zurück nach oben
Bilder des Herstellers
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) antibody (PE-Atto 594) (ABIN2481441) Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp60 Monoclonal Ant...
Immunohistochemistry (IHC) image for anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) antibody (PE-Atto 594) (ABIN2481441) Immunohistochemistry analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-1 ...
Western Blotting (WB) image for anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) antibody (PE-Atto 594) (ABIN2481441) Western Blot analysis of Human Cell line lysates showing detection of Hsp60 protein u...
Immunohistochemistry (IHC) image for anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) antibody (PE-Atto 594) (ABIN2481441) Immunohistochemistry analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-1 ...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) antibody (PE-Atto 594) (ABIN2481441) Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp60 Monoclonal Ant...