IFNB1 Protein

Produktnummer ABIN935242

Recombinant IFNB1-Protein exprimiert in CHO Cells.

Kurzübersicht für IFNB1 Protein (ABIN935242)

Target: IFNB1 (Interferon, beta 1, Fibroblast (IFNB1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: CHO Cells

Reinheit: > 95 % pure

Produktdetails

Sequenz: MSYNLLGFLQ RSSNFQCQKL LWQLNGRLEY CLKDRMNFDI PEEIKQLQQF QKEDAALTIY EMLQNIFAIF RQDSSSTGWN ETIVENLLAN VYHQINHLKT VLEEKLEKED FTRGKLMSSL HLKRYYGRIL HYLKAKEYSH CAWTIVRVEI LRNFYFINRL TGYLRN

Produktmerkmale: Purified recombinant Human IFN beta protein
Expression System: CHO cells
Bioactivity: Assay#1: The biological activity of PeproTech's human IFN-gamma was based on analysis using the WISH cell line with VSV as the challenge virus. The specific activitywas determined to be >5 x 10 8 units/mg, Assay#2: Measured by its ability to induce apoptosis in HeLa cells. The expected ED50 for this effect is 20-30 ng/mL.

Endotoxin-Niveau: < 0.1 ng per µg (1 EU/µg).

Anwendungsinformationen

Applikationshinweise: Each Investigator should determine their own optimal working dilution for specific applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute in water to a concentration of 0.1-1.0 g/mL. Do not vortex. For extended storage, dilute further in a buffer containing a carrier protein (ie: 0.1 % BSA).

Buffer: Lyophilized from 10 mM sodium citrate, with 100 mM NaCl, and 10 mM Sucrose.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.

Antigendetails

Target: IFNB1 (Interferon, beta 1, Fibroblast (IFNB1))

Andere Bezeichnung: IFN beta

Hintergrund: Proteins of this family play an important role in inducing non-specific resistance against a broad range of viral infections. They also affect cell proliferation and modulate immune responses. Produced by peripheral blood leukocytes and lymphoblastoid cells, IFNa is an acid stable molecule that signals through IFNa/betaR, which is also used by IFNbeta. Both IFNs have similar anti-viral activity and regulate expression of MHC class I antigens. IFNa contains four highly conserved cysteine residues which form two disulfide bonds, one of which is necessary for biological activity.
Alternative Names: Leukocyte interferon antibody, RMMB1 antibody, Interferon beta antibody, Type I interferon antibody, B cell interferon antibody

Molekulargewicht: 20 kDa

Pathways: JAK-STAT Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Autophagie, Inflammasome