IL-6 Protein (AA 25-211)

Produktnummer ABIN7321248

Dieses Recombinant IL-6-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für IL-6 Protein (AA 25-211) (ABIN7321248)

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Ratte

Quelle: Escherichia coli (E. coli)

Applikation: Cell Culture (CC)

Reinheit: > 90 % as determined by reducing SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 25-211

Verwendungszweck: Recombinant Rat IL-6/Interleukin-6 Protein

Sequenz: Phe25-Thr211

Produktmerkmale: A DNA sequence encoding the rat IL6 (Phe25-Thr211) was expressed and purified with an initial Met.

Sterilität: 0.2 μm filtered

Biological Activity Comment: Measured in a cell proliferation assay using T1165 mouse plasmacytoma cells. The ED₅₀ for this effect is typically 1-6 ng/ml.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from sterile 40 % acetonitrile, 0.1 % TFA
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Andere Bezeichnung: IL-6/Interleukin-6

Hintergrund: IL6,Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. It exerts actions through the its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

Molekulargewicht:

Calculated MW: 21.9 kDa

Observed MW: 22 kDa

NCBI Accession: NP_036721

UniProt: A6KJN3

Pathways: TLR Signalweg, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagie, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome