NGFB Protein

Produktnummer ABIN6700287

Hersteller Produkt- Nr.: 009-001-w03-0020

Recombinant NGFB-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für NGFB Protein (ABIN6700287)

Target: NGFB (Nerve Growth Factor beta (NGFB))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: 97,00%

Produktdetails

Hersteller Produkt- Nr.: 009-001-w03-0020

Hersteller: Rockland

Verwendungszweck: Human Nerve Growth Factor beta Recombinant Protein

Aufreinigung: Nerve Growth Factor beta purity was determined to be greater than 97% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

Endotoxin-Niveau: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the ability to stimulate proliferation of TF-1 cells and is typically less than 1 ng/mL.

Anwendungsinformationen

Applikationshinweise:

Application Note: Nerve Growth Factor beta Recombinant Protein is suitable as a control for polyclonal or monoclonal anti-Nerve Growth Factor beta in immunological assays.

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 20 μL (20-200 μL)

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Antigendetails

Target: NGFB (Nerve Growth Factor beta (NGFB))

Andere Bezeichnung: NGF beta

Hintergrund:

Synonyms: β-NGF

Background: Nerve Growth Factor (NGF-β) is a neurotrophic factor related to BDNF, NT-3 and NT-4. NGF-β acts through its receptor β-NGFR, and is involved in the development and maintenance of the sensory and sympathetic nervous systems. NGF-β is also involved in the growth, differentiation and survival of B lymphocytes. Human, mouse and rat proteins show cross-reactivity. Recombinant human NGF-β is a non-covalently linked homodimer, containing two 121 amino acid chains, with a total molecular weight of 27.2 kDa.

UniProt: P01138

Pathways: NF-kappaB Signalweg, RTK Signalweg, Regulation of Cell Size