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Cathepsin B Protein (CTSB) (AA 18-339) (His tag)

CTSB Spezies: Maus Wirt: Baculovirus infected Insect Cells Recombinant > 90% by SDS-PAGE SDS, EAA Active
Produktnummer ABIN6387936
  • Target Alle Cathepsin B (CTSB) Proteine anzeigen
    Cathepsin B (CTSB)
    Protein-Typ
    Recombinant
    Biologische Aktivität
    Active
    Proteineigenschaft
    AA 18-339
    Spezies
    • 17
    • 6
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus
    Quelle
    • 10
    • 7
    • 6
    • 4
    • 2
    • 2
    • 1
    Baculovirus infected Insect Cells
    Aufreinigungstag / Konjugat
    Dieses Cathepsin B Protein ist gelabelt mit His tag.
    Applikation
    SDS-PAGE (SDS), Enzyme Activity Assay (EAA)
    Sequenz
    HDKPSFHPLS DDLINYINKQ NTTWQAGRNF YNVDISYLKK LCGTVLGGPK LPGRVAFGED IDLPETFDAR EQWSNCPTIG QIRDQGSCGS CWAFGAVEAI SDRTCIHTNG RVNVEVSAED LLTCCGIQCG DGCNGGYPSG AWSFWTKKGL VSGGVYNSHV GCLPYTIPPC EHHVNGSRPP CTGEGDTPRC NKSCEAGYSP SYKEDKHFGY TSYSVSNSVK EIMAEIYKNG PVEGAFTVFS DFLTYKSGVY KHEAGDMMGG HAIRILGWGV ENGVPYWLAA NSWNLDWGDN GFFKILRGEN HCGIESEIVA GIPRTDQYWG RFLEHHHHHH
    Reinheit
    > 90% by SDS-PAGE
    Endotoxin-Niveau
    < 1 EU per 1ug of protein (determined by LAL method)
    Biological Activity Comment
    Specific activity is > 2,000pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of Z-Arg-Arg-AMC to Z-Arg-Arg and AMC per minute at pH 6.0 at 37C.
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  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Kommentare

    Bioactivity Validated

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.5 mg/mL
    Buffer
    Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 10 % glycerol.
    Lagerung
    4 °C,-20 °C,-80 °C
    Informationen zur Lagerung
    Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
  • Target
    Cathepsin B (CTSB)
    Andere Bezeichnung
    Cathepsin B (CTSB Produkte)
    Hintergrund
    CTSB, also known as cathepsin B preproprotein, is a papain-family cysteine protease that is normally located in lysosomes. It has the ability to degrade several extracellular matrix components at both neutral and acidic pH and has been implicated in the progression of several human and rodent tumors progression and arthritis. Recombinant mouse CTSB, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
    Molekulargewicht
    36.4 kDa (330aa)
    NCBI Accession
    NP_031824
    UniProt
    P10605
    Pathways
    Activation of Innate immune Response, Toll-Like Receptors Cascades
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