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Cathepsin B (CTSB) (AA 18-339) protein (His tag) Protein

CTSB Spezies: Human Wirt: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
Pubmed (2 Referenzen)
Produktnummer ABIN2180942
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  • Target Alle Cathepsin B (CTSB) Proteine anzeigen
    Cathepsin B (CTSB)
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 18-339
    Spezies
    • 17
    • 8
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Quelle
    • 7
    • 7
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    HEK-293 Cells
    Aufreinigungstag / Konjugat
    Dieses Cathepsin B Protein ist gelabelt mit His tag.
    Sequenz
    AA 18-339
    Produktmerkmale
    rh CTSB is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 36.7 kDa (pro-form) and 29 kDa (mature-form). The predicted N-terminus is Arg18 (pro-form) or Phe74 (mature-form). Due to glycosylation, The reducing (R) protein migrates as 43 kDa (pro-form) band in SDS-PAGE and there may be a 34 kDa (mature-form) band.
    Reinheit
    >95 % as determined by SDS-PAGE.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Less than 1.0 EU per μg by the LAL method.
  • Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Buffer
    50 mM Tris, 150 mM NaCl, pH 8.0
    Handhabung
    Please avoid repeated freeze-thaw cycles.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
  • Hartmann, Lopez Cruz, Alameh, Ho, Rabideau, Pentelute, Bradley, Martchenko: "Characterization of Novel Piperidine-Based Inhibitor of Cathepsin B-Dependent Bacterial Toxins and Viruses." in: ACS infectious diseases, Vol. 4, Issue 8, pp. 1235-1245, (2018) (PubMed).

    Zilbermintz, Leonardi, Jeong, Sjodt, McComb, Ho, Retterer, Gharaibeh, Zamani, Soloveva, Bavari, Levitin, West, Bradley, Clubb, Cohen, Gupta, Martchenko: "Identification of agents effective against multiple toxins and viruses by host-oriented cell targeting." in: Scientific reports, Vol. 5, pp. 13476, (2015) (PubMed).

  • Target
    Cathepsin B (CTSB)
    Andere Bezeichnung
    Cathepsin B (CTSB Produkte)
    Synonyme
    APPS, CPSB, CB, apps, cpsb, LOC692390, CTSB, cathepsin B, cathepsin B S homeolog, CTSB, Ctsb, ctsb.S, LOC692390
    Hintergrund
    Cathepsin B (CTSB) is also known as APP secretase (APPS) and CPSB, is an enzymatic protein belonging to the peptidase C1 family. Cathepsin B / CTSB is synthesized as a preproenzyme. Following removal of the signal peptide, the inactive proenzyme undergoes further modifications including removal of the pro region to result in the active enzyme. The catalytic activity of Cathepsin B / APPS contains: Hydrolysis of proteins with broad specificity for peptide bonds, Preferentially cleaves -Arg-Arg-|-Xaa bonds in small molecule substrates (thus differing from cathepsin L), In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides. As a thiol protease, cathepsin B / CPSB is believed to participate in intracellular degradation and turnover of proteins and has also been implicated in tumor invasion and metastasis. Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors.
    Molekulargewicht
    36.7 kDa & 29.5 kDa
    NCBI Accession
    NP_001899
    Pathways
    Activation of Innate immune Response, Toll-Like Receptors Cascades
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