Interleukin 12 (IL12) (AA 23-219), (AA 23-328) (Active) Protein

Details zu Produkt Nr. ABIN2018013
Proteinname
Proteineigenschaft
AA 23-219, AA 23-328
8
6
5
3
2
2
1
1
1
1
1
Spezies
Human
35
18
8
1
1
1
1
1
1
1
Quelle
HEK-293T Cells
20
13
7
7
5
2
2
2
1
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Optionen
Produktmerkmale EC50 < 2 ng/mL, measured by the induction of IFN-gamma from NK cells co-stimulated with IL-18, corresponding to a specific activity of > 1x10^6 units/mg.
P35 subunit: AA 23-219 ,P40 subunit: AA 23-328
Reinheit > 95 % as analyzed by SDS-PAGE and HPLC.
Endotoxin-Niveau < 0.2 EU/μg, determined by LAL method.
Proteinname
Hintergrund Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF. These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and mouse IL-12 share 70 % and 60 % amino acid sequence homology in their p40 and p35 subunits, respectively. IL-12 apparently shows species specificity with human IL-12 reportedly showing minimal activity in the mouse system.
Synonyms: NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF
Molekulargewicht 75 kDa, consisting of a 306 amino acid residue p40 subunit and a 197 amino acid residue p35 subunit.
UniProt P29459, P29460
Pathways JAK-STAT Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Reconstituted in ddH2O or PBS at 100 μg/mL.
Buffer Lyophilized after extensive dialysis against PBS.
Lagerung -80 °C
Informationen zur Lagerung Lyophilized recombinant Human Interleukin 12(IL-12) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rh-IL12 should be stable up to 1 week at 4 °C or up to 2 months at -20 °C.
Haltbarkeit 6 months
Bilder des Herstellers
Image no. 1 for Interleukin 12 (IL12) (AA 23-219), (AA 23-328) (Active) protein (ABIN2018013) IL-12, Human induction of IFN-γ from NK cells co-stimulated with IL-18. The ED50 for ...
Image no. 2 for Interleukin 12 (IL12) (AA 23-219), (AA 23-328) (Active) protein (ABIN2018013) Interleukin 12 (IL12) (AA 23-219), (AA 23-328) (Active) protein (Image 2)
Image no. 3 for Interleukin 12 (IL12) (AA 23-219), (AA 23-328) (Active) protein (ABIN2018013) 2 μg of IL-12, Human was resolved with SDS-PAGE under reducing (R) and non-reducing (...