Bcl-2 Protein (AA 1-239) (GST tag)
(1 reference)-
- Target Alle Bcl-2 (BCL2) Proteine anzeigen
- Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 1-239
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Spezies
- Human
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Quelle
- Wheat germ
- Aufreinigungstag / Konjugat
- Dieses Bcl-2 Protein ist gelabelt mit GST tag.
- Applikation
- Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)
- Verwendungszweck
- BCL2 (Human) Recombinant Protein (P01)
- Sequenz
- MAHAGRTGYD NREIVMKYIH YKLSQRGYEW DAGDVGAAPP GAAPAPGIFS SQPGHTPHPA ASRDPVARTS PLQTPAAPGA AAGPALSPVP PVVHLTLRQA GDDFSRRYRR DFAEMSSQLH LTPFTARGRF ATVVEELFRD GVNWGRIVAF FEFGGVMCVE SVNREMSPLV DNIALWMTEY LNRHLHTWIQ DNGGWDAFVE LYGPSMRPLF DFSWLSLKTL LSLALVGACI TLGAYLGHK
- Produktmerkmale
- Human BCL2 full-length ORF ( AAH27258.1, 1 a.a. - 239 a.a.) recombinant protein with GST-tag at N-terminal.
- Aufreinigung
- in vitro wheat germ expression system
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B-Cell CLL/lymphoma 2 (BCL2) (AA 5-205) protein (His tag)
BCL2 Spezies: Maus Wirt: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
B-Cell CLL/lymphoma 2 (BCL2) (AA 1-211) protein (His-IF2DI Tag)BCL2 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant Greater than 90 % by SDS-PAGE gel analyses WB, ELISA
B-Cell CLL/lymphoma 2 (BCL2) (AA 140-239) protein (GST tag)BCL2 Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AA, AP
B-Cell CLL/lymphoma 2 (BCL2) (AA 1-211) protein (His tag)BCL2 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant > 90 % by SDS - PAGE SDS
B-Cell CLL/lymphoma 2 (BCL2) (AA 1-236) protein (His tag)custom-made BCL2 Spezies: Maus Wirt: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Kommentare
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handhabung
- Aliquot to avoid repeated freezing and thawing.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Best use within three months from the date of receipt of this protein.
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-
: "Threonine 56 phosphorylation of Bcl-2 is required for LRRK2 G2019S-induced mitochondrial depolarization and autophagy." in: Biochimica et biophysica acta, Vol. 1852, Issue 1, pp. 12-21, (2014) (PubMed).
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: "Threonine 56 phosphorylation of Bcl-2 is required for LRRK2 G2019S-induced mitochondrial depolarization and autophagy." in: Biochimica et biophysica acta, Vol. 1852, Issue 1, pp. 12-21, (2014) (PubMed).
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- Target
- Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))
- Andere Bezeichnung
- BCL2 (BCL2 Produkte)
- Hintergrund
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Full Gene Name: B-cell CLL/lymphoma 2
Synonyms: Bcl-2 - Gen-ID
- 596
- Pathways
- MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagie, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling
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