c-MYC Protein (AA 1-439) (His tag)

Produktnummer ABIN1047411

Produktdetails

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-439

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses c-MYC Protein ist gelabelt mit His tag.

Applikation: ELISA, SDS-PAGE (SDS)

Sequenz: MPLNVSFTNR NYDLDYDSVQ PYFYCDEEEN FYQQQQQSEL QPPAPSEDIW KKFELLPTPP LSPSRRSGLC SPSYVAVTPF SLRGDNDGGG GSFSTADQLE MVTELLGGDM VNQSFICDPD DETFIKNIII QDCMWSGFSA AAKLVSEKLA SYQAARKDSG SPNPARGHSV CSTSSLYLQD LSAAASECID PSVVFPYPLN DSSSPKSCAS QDSSAFSPSS DSLLSSTESS PQGSPEPLVL HEETPPTTSS DSEEEQEDEE EIDVVSVEKR QAPGKRSESG SPSAGGHSKP PHSPLVLKRC HVSTHQHNYA APPSTRKDYP AAKRVKLDSV RVLRQISNNR KCTSPRSSDT EENVKRRTHN VLERQRRNEL KRSFFALRDQ IPELENNEKA PKVVILKKAT AYILSVQAEE QKLISEEDLL RKRREQLKHK LEQLRNSCA

Produktmerkmale: Recombinant full-length human Myc proto-oncogene protein (MYC) cDNA (439 aa) constructed with a 6xHis-tag at the N-terminus was expressed in E. coli. It got purified up to 90% as determined by SDS-PAGE. Its predicted molecular weight is 52.8 kDa, but the observed is about 60 kDa. Posttranslational modification results in the higher actual molecular weight.

Reinheit: Greater than 90% as determined by SDS-PAGE.

Anwendungsinformationen

Applikationshinweise: This MYC protein may find uses on specific antibody production, iPS generation mechanism mediated by human MYC in vitro, or related signaling transduction.

Kommentare:

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%.

Buffer: If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Handhabung: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Referenzen

Bernard, Cory, Gerondakis, Webb, Adams: "Sequence of the murine and human cellular myc oncogenes and two modes of myc transcription resulting from chromosome translocation in B lymphoid tumours." in: The EMBO journal, Vol. 2, Issue 12, pp. 2375-83, (1984) (PubMed).

Battey, Moulding, Taub, Murphy, Stewart, Potter, Lenoir, Leder: "The human c-myc oncogene: structural consequences of translocation into the IgH locus in Burkitt lymphoma." in: Cell, Vol. 34, Issue 3, pp. 779-87, (1983) (PubMed).

Colby, Chen, Smith, Levinson: "Identification and nucleotide sequence of a human locus homologous to the v-myc oncogene of avian myelocytomatosis virus MC29." in: Nature, Vol. 301, Issue 5902, pp. 722-5, (1983) (PubMed).

Antigendetails

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Abstract: MYC Produkte

Hintergrund: Synonyms: Class E basic helix-loop-helix protein 39,bHLHe39,Proto-oncogene c-Myc,Transcription factor p64
MYC is an oncoprotein that plays a central role in almost the whole oncogenic process, orchestrating proliferation, apoptosis, differentiation, and metabolism. As a transcription factor, MYC regulates transcription of numerous specific target genes that are involved in distinct cellular functions, such as cell cycle, protein biosynthesis, cell adhesion and cytoskeleton, metabolism, signal transduction, transcription, and translation. Aberrations in MYC protein, including mutations, overexpression, rearrangement, and translocation, have been linked to various hematopoietic tumors, leukemias, and lymphomas.

Molekulargewicht: 52.8 kDa

UniProt: P01106

Pathways: p53 Signalweg, Zellzyklus, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Warburg Effekt