Insulin ELISA Kit

Produktnummer ABIN8081141

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human, Ratte, Maus und Hund Insulin.

Kurzübersicht für Insulin ELISA Kit (ABIN8081141)

Target: Insulin (INS)

Reaktivität: Human, Ratte, Maus, Hund

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 6.17 pg/mL - 500 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Produktdetails Insulin ELISA Kit

Untere Nachweisgrenze: 6.17 pg/mL

Verwendungszweck: Human/Mouse/Rat/Dog Insulin (INS) ELISA Kit for detecting INS in serum, plasma, tissue homogenates, cell lysates, cell culture supernates

Analytische Methode: Quantitative

Sensitivität: 2.74 pg/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 50 μL

Testdauer: 2 h

Plattentyp: Pre-coated

Testpräzision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 12 months

Details zu Insulin

Target: Insulin (INS)

Andere Bezeichnung: Insulin

Hintergrund: Gene Aliases:

Pathways: NF-kappaB Signalweg, RTK Signalweg, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagie, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation