PRAS40 ELISA Kit

Produktnummer ABIN8074236

Das Human, Maus und Ratte PRAS40 ELISA Kit (ABIN8074236) ist ein Colorimetric ELISA Kit zur Detektion von Human, Maus und Ratte PRAS40.

Kurzübersicht für PRAS40 ELISA Kit (ABIN8074236)

Target: PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

Bindungsspezifität: pThr246

Reaktivität: Human, Maus, Ratte

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Culture Cells

Produktdetails PRAS40 ELISA Kit

Verwendungszweck: Phospho-Akt1 S1 (Thr246) Colorimetric Cell-Based ELISA Kit for detecting Phospho-Akt1 S1 (Thr246) in researchers plate their cell line of choice.

Analytische Methode: Qualitative

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Testdauer: 4 - 5 h

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 6 months

Details zu PRAS40

Target: PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

Andere Bezeichnung: Akt1 S1

Hintergrund: Gene Aliases: Akt1 substrate 1 (proline-rich) AKTS1, Lobe, MGC2865, PRAS40, proline-rich Akt substrate 40 kDa

Gen-ID: 84335

UniProt: Q96B36

Pathways: Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Cell Size, Autophagie, BCR Signaling, Warburg Effekt