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PRAS40 ELISA Kit

AKT1S1 Reaktivität: Human pThr246 Colorimetric Cell ELISA Adherent Cell Culture
Produktnummer ABIN5526720
  • Target Alle PRAS40 (AKT1S1) ELISA Kits anzeigen
    PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))
    Bindungsspezifität
    pThr246, total
    Reaktivität
    • 3
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Cell ELISA
    Applikation
    ELISA
    Verwendungszweck
    Cell-Based ELISA Kit. This assay semi-quantitatively measures PRAS40 phosphorylated at Threonine-246 as well as total PRAS40 in adherent cell lines.
    Proben
    Adherent Cell Culture
    Analytische Methode
    Semi-Quantitative
    Spezifität
    This ELISA kit recognizes Human PRAS40 phosphorylated at site Threonine-246 as well as total PRAS40.
    Produktmerkmale
    • Rapidly measure phosphorylated protein in adherent cell lines
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • No sample lysis is needed
    • Compatible with a standard ELISA plate reader
    Bestandteile
    • Uncoated 96-well Strip Microplate
    • Wash Buffers
    • Fixing Solution
    • Quenching Buffer
    • Blocking Buffer
    • Anti-phospho antibody
    • Anti-pan antibody
    • HRP-Conjugated Secondary Antibody
    • TMB One-Step Substrate
    • Stop Solution
    Benötigtes Material
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Top Product
    Discover our top product AKT1S1 ELISA Kit
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Haltbarkeit
    6 months
  • Target Alle PRAS40 (AKT1S1) ELISA Kits anzeigen
    PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))
    Andere Bezeichnung
    PRAS40 (AKT1S1 Produkte)
    Synonyme
    MGC81452 ELISA Kit, lobe ELISA Kit, pras40 ELISA Kit, fb34a04 ELISA Kit, wu:fb34a04 ELISA Kit, Lobe ELISA Kit, PRAS40 ELISA Kit, 1110012J22Rik ELISA Kit, AI227026 ELISA Kit, Lobel ELISA Kit, AKT1 substrate 1 (proline rich) S homeolog ELISA Kit, AKT1 substrate 1 ELISA Kit, AKT1 substrate 1 (proline rich) ELISA Kit, AKT1 substrate 1 (proline-rich) ELISA Kit, akt1s1.S ELISA Kit, AKT1S1 ELISA Kit, akt1s1 ELISA Kit, Akt1s1 ELISA Kit
    Gen-ID
    84335
    UniProt
    Q96B36
    Pathways
    Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Cell Size, Autophagie, BCR Signaling, Warburg Effekt
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