Insulin ELISA Kit

Produktnummer ABIN8069778

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human Insulin.

Kurzübersicht für Insulin ELISA Kit (ABIN8069778)

Target: Insulin (INS)

Bindungsspezifität: Oxidized Form, total

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Proben: Cell Lysate, Tissue Lysate

Produktdetails Insulin ELISA Kit

Verwendungszweck: Measures oxidated Insulin (INS) and total Insulin in human cell and tissue lysates, enabling precise assessment of Insulin oxidation by ELISA. This assay can be developed for other species (mouse, rat, etc.) upon request. Contact techsupport@raybiotech.com.

Analytische Methode: Quantitative

Bestandteile:

Size / Qty
• Microplate Coated With Anti-Pan-Insulin Antibody
• Positive Control
• HRP-Conjugated Streptavidin
• 200 μL
• Wash Buffer
• Assay Diluent
• Lysis Buffer
• TMB One-Step Substrate Reagent
• Stop Solution
• Oxidation Buffer A
• Oxidation Buffer B
• Oxidation Labeling Reagent
• Oxidation Stabilizing Reagent

Benötigtes Material:

• 20 % (v/v) trichloroacetic acid (TCA) solution, ice-cold
• 1:1 (v/v) ethanol:ethyl acetate
• Benchtop centrifuge and microcentrifuge (4 °C)
• Microplate reader capable of measuring absorbance at 450 nm
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Log-log graph paper or computer and software for ELISA data analysis
• Absorbent paper
• Tubes to prepare positive control or sample dilutions
• Distilled or deionized water
• Shaker

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and Positive Control as instructed.
2. Label samples with Oxidation Labeling Reagent
3. Add Oxidation Stabilizing Reagent to samples.
4. Precipitate proteins with ice-cold 20 % TCA.
5. Wash protein pellet twice with 1:1 (v/v) ethanol:ethyl acetate.
6. Resuspend protein pellets in Oxidation Buffer B.
7. Add 100 μL sample and Positive Control to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C with gentle shaking.
8. Add 100 μL prepared HRP-Conjugated Streptavidin solution. Incubate for 1 hour at room temperature with gentle shaking.
9. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
10. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

Details zu Insulin

Target: Insulin (INS)

Andere Bezeichnung: Insulin

Hintergrund:

Gene Symbols: INS

Background: The Oxidated Insulin ELISA Kit uses the "biotin probes" method to biotin label carbonyl groups of oxidized proteins. In this method, the biotin probes first react with carbonyl groups of oxidized proteins to form unstable Schiff bases, which are then further reduced to more stable amines. The biotinylated proteins can then be detected by ELISA. An anti-pan Insulin antibody has been coated onto a 96-well plate. Biotinylated samples are pipetted into the wells, and Insulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and HRP-conjugated streptavidin is used to detect oxidated Insulin. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of oxidated Insulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Protein Name (Synonyms): Insulin

Gen-ID: 3630

UniProt: P01308

Pathways: NF-kappaB Signalweg, RTK Signalweg, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagie, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation