MAPK14 ELISA Kit
Kurzübersicht für MAPK14 ELISA Kit (ABIN7830792)
Target
Alle MAPK14 ELISA Kits anzeigenReaktivität
Nachweismethode
Methodentyp
Applikation
Proben
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Verwendungszweck
- MAPK14 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of MAPK14 expression in cultured cells.
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Analytische Methode
- Qualitative
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Kreuzreaktivität
- Maus, Ratte
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Plattentyp
- Uncoated
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Protokoll
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Lagerung
- 4 °C
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Informationen zur Lagerung
- Store the kit at 4°C.
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- MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))
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Andere Bezeichnung
- MAPK14
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Hintergrund
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Synonyms: CSBP1|CSBP2|CSPB1|EXIP|Mxi2|PRKM14|PRKM15|RK|SAPK2A|p38|p38ALPHA
Gene Description: mitogen-activated protein kinase 14
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Gen-ID
- 1432
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Pathways
- MAPK Signalweg, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Hepatitis C, Toll-Like Receptors Cascades, Autophagie, Thromboxane A2 Receptor Signaling, BCR Signaling, S100 Proteine
Target Alle MAPK14 ELISA Kits anzeigen
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