JNK ELISA Kit

Produktnummer ABIN7830695

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human JNK.

Kurzübersicht für JNK ELISA Kit (ABIN7830695)

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails JNK ELISA Kit

Verwendungszweck: MAPK8/MAPK9/MAPK10 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of MAPK8/MAPK9/MAPK10 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu JNK

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Andere Bezeichnung: MAPK8

Hintergrund:

Synonyms: JNK|JNK1|JNK1A2|JNK21B1/2|PRKM8|SAPK1

Gene Description: mitogen-activated protein kinase 8

Gen-ID: 5599

UniProt: P45983

Pathways: MAPK Signalweg, WNT Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, S100 Proteine