Insulin IQ-ELISA Kit

Produktnummer ABIN6384302

Produktdetails Insulin IQ-ELISA Kit

Target: Insulin (INS)

Reaktivität: Maus

Nachweismethode: qPCR

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Mouse Immunoquantitative (PCR-Based) Insulin ELISA Kit for cell culture supernatants, plasma, and serum samples.

Proben: Cell Culture Supernatant, Plasma, Serum

Analytische Methode: Semi-Quantitative

Sensitivität: 0.5 mIU/mL

Produktmerkmale: IQ-ELISAs employ specific capture antibodies coated on a 96-well PCR plate. Standards and samples are pipetted into the wells, the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the detection affinity reagent is added to the wells where it binds to any captured antigen. The wells are washed, and primers and PCR master mix are added to each well. The plate is placed into a real time PCR instrument for cycling and measurement of DNA amplification. The cycle number where amplification is detected is proportional to the amount of affinity detection reagent that bound to captured antigen in each well.

Bestandteile:

• Insulin Microplate (Item A): 96 well PCR plate coated with anti-Mouse Insulin
• Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
• Standards (Item C): 2 vials of recombinant Mouse Insulin
• Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
• Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
• Detection Affinity Reagent for Insulin (Item F): 2 vials of a 4x concentrated solution of anti-Mouse Insulin affinity reagent
• IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
• Primer Solution (Item I): 1.7 ml vial
• PCR Master Mix (Item J): 1.2 ml vial
• PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
• Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer

Benötigtes Material:

• Real-time PCR instrument, Bio-Rad recommended
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Log-log graph paper or computer and software for data analysis
• Tubes to prepare standard or sample dilutions
• Heating block or water bath capable of 80^°C

Anwendungsinformationen

Applikationshinweise: The Immuno-Quantitative ELISA (IQELISA) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.

Kommentare:

The Immuno-Quantitative ELISA (IQELISA™) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. Also called immuno-PCR, this detection platform results in an assay that is simultaneously familiar and cutting edge. Compared to traditional ELISA, IQELISA™ enables the use of only 1/10th the sample volume while also providing 10x more sensitivity.

Probenmenge: 25 µL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed
2. Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4^°C
3. Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
4. Add 25μL of IQELISA Detection Reagent to each well. Incubate 1 hour
5. Add 15μL Primer solution 10μL of PCR master mix to each well
6. Run real-time PCR

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 6 months

Antigendetails

Target: Insulin (INS)

Andere Bezeichnung: Insulin (INS Produkte)

Synonyme: IDDM2 IQ-ELISA Kit, ILPR IQ-ELISA Kit, IRDN IQ-ELISA Kit, MODY10 IQ-ELISA Kit, ins1 IQ-ELISA Kit, xins IQ-ELISA Kit, ins1-a IQ-ELISA Kit, Insulin IQ-ELISA Kit, AA986540 IQ-ELISA Kit, Ins-2 IQ-ELISA Kit, InsII IQ-ELISA Kit, Mody IQ-ELISA Kit, Mody4 IQ-ELISA Kit, proinsulin IQ-ELISA Kit, zgc:109842 IQ-ELISA Kit, igf2-A IQ-ELISA Kit, ins IQ-ELISA Kit, ins-a IQ-ELISA Kit, ins-b IQ-ELISA Kit, insulin IQ-ELISA Kit, insulin precursor IQ-ELISA Kit, insulin II IQ-ELISA Kit, preproinsulin IQ-ELISA Kit, insulin L homeolog IQ-ELISA Kit, insulin S homeolog IQ-ELISA Kit, INS IQ-ELISA Kit, INS-IGF2 IQ-ELISA Kit, ins IQ-ELISA Kit, Ins IQ-ELISA Kit, PIN IQ-ELISA Kit, Ins2 IQ-ELISA Kit, ins.L IQ-ELISA Kit, ins.S IQ-ELISA Kit

Gen-ID: 16333

UniProt: P01325

Pathways: NF-kappaB Signalweg, RTK Signalweg, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagie, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation