TNF alpha ELISA Kit

Produktnummer ABIN6210920

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Huhn TNF alpha.

Kurzübersicht für TNF alpha ELISA Kit (ABIN6210920)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reaktivität: Huhn

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2 pg/mL - 2000 pg/mL

Applikation: ELISA

Proben: Cell Lysate, Plasma, Serum, Tissue Homogenate

Produktdetails TNF alpha ELISA Kit

Untere Nachweisgrenze: 31.2 pg/mL

Verwendungszweck: Chicken Tumor Necrosis Factor (TNF) ELISA Kit

Analytische Methode: Quantitative

Sensitivität: 18.8 pg/mL

Bestandteile: The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

• Pre-coated 96-Well Microplate
• Standard
• Standard Diluent Buffer
• Wash Buffer
• Detection Reagent A
• Detection Reagent B
• Diluent A
• Diluent B
• TMB Substrate
• Stop Solution
• Plate Sealer

Benötigtes Material:

• 37 °C incubator
• Multi and single channel pipettes and sterile pipette tips
• Squirt bottle or automated microplate washer
• 1.5 mL tubes
• Distilled water
• Absorbent filter papers
• 100 mL and 1 liter graduated cylinders
• Microplate reader (wavelength: 450 nm)
• ELISA Shaker

Anwendungsinformationen

Applikationshinweise: Optimal dilutions/concentrations should be determined by the end user.

Kommentare:

The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5 % within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.

Plattentyp: Pre-coated

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of Tumor Necrosis Factor Alpha (TNFA) were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of Tumor Necrosis Factor Alpha (TNFA) were tested on 3 different plates, 8 replicates in each plate.

CV (%) = (Standard Deviation / mean) x 100

Intra-Assay: CV<10%

Inter-Assay: CV<10%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.

Haltbarkeit: 6 months

Details zu TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Andere Bezeichnung: Tumor Necrosis Factor

Hintergrund: F1DFK9_CHICK

UniProt: F1DFK9

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome