CHEK1 ELISA Kit

Produktnummer ABIN5652789

Produktdetails CHEK1 ELISA Kit

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Checkpoint Kinase 1 (CHEK1). No significant cross-reactivity or interference between Checkpoint Kinase 1 (CHEK1) and analogues was observed.

Sensitivität: 0.058 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Checkpoint Kinase 1 (CHEK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Checkpoint Kinase 1 (CHEK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Checkpoint Kinase 1 (CHEK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Checkpoint Kinase 1 (CHEK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Checkpoint Kinase 1 (CHEK1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Checkpoint Kinase 1 (CHEK1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu CHEK1

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Andere Bezeichnung: Checkpoint Kinase 1 (CHEK1 Produkte)

Synonyme: An08g10320 ELISA Kit, AO090003000441 ELISA Kit, CHK1 ELISA Kit, chk1 ELISA Kit, C85740 ELISA Kit, Chk1 ELISA Kit, rad27 ELISA Kit, id:ibd2720 ELISA Kit, zgc:56093 ELISA Kit, CHEK1 ELISA Kit, checkpoint kinase 1 ELISA Kit, serine/threonine-protein kinase chk1 ELISA Kit, serine/threonine-protein kinase Chk1 ELISA Kit, CAMK/CAMKL/CHK1 protein kinase Chk1 ELISA Kit, checkpoint kinase 1 S homeolog ELISA Kit, CHEK1 ELISA Kit, ANI_1_2436074 ELISA Kit, AOR_1_768154 ELISA Kit, PTRG_04183 ELISA Kit, SJAG_01680 ELISA Kit, PAAG_04978 ELISA Kit, MCYG_03290 ELISA Kit, VDBG_03742 ELISA Kit, chek1.S ELISA Kit, Chek1 ELISA Kit, chek1 ELISA Kit

Hintergrund:

Gene Name: Checkpoint Kinase 1

Gene Aliases: CHK1, Cell cycle checkpoint kinase, Serine/threonine-protein kinase Chk1

Gen-ID: 12649

UniProt: O35280

Pathways: p53 Signalweg, Apoptose, Zellzyklus, DNA Reparatur