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CHEK1 ELISA Kit

CHEK1 Reaktivität: Human pSer280 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Produktnummer ABIN4886023
  • Target Alle CHEK1 ELISA Kits anzeigen
    CHEK1 (Checkpoint Kinase 1 (CHEK1))
    Bindungsspezifität
    pSer280, total
    Reaktivität
    • 8
    • 3
    • 2
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human Phospho-Chk1 (S280) and Total Chk1 ELISA Kit. This assay semi-quantitatively measures phophorylated Chk1 (Ser280) and Total Chk1 in lysate samples.
    Proben
    Cell Lysate, Tissue Lysate
    Analytische Methode
    Semi-Quantitative
    Spezifität
    The antibody pair provided in this kit recognizes human Chk1 phosphorylated at site Serine-280 and total Chk1
    Produktmerkmale
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Benötigtes Material
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Top Product
    Discover our top product CHEK1 ELISA Kit
  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Haltbarkeit
    6 months
  • Target Alle CHEK1 ELISA Kits anzeigen
    CHEK1 (Checkpoint Kinase 1 (CHEK1))
    Andere Bezeichnung
    CHK1 (CHEK1 Produkte)
    Synonyme
    An08g10320 ELISA Kit, AO090003000441 ELISA Kit, CHK1 ELISA Kit, chk1 ELISA Kit, C85740 ELISA Kit, Chk1 ELISA Kit, rad27 ELISA Kit, id:ibd2720 ELISA Kit, zgc:56093 ELISA Kit, CHEK1 ELISA Kit, checkpoint kinase 1 ELISA Kit, serine/threonine-protein kinase chk1 ELISA Kit, serine/threonine-protein kinase Chk1 ELISA Kit, CAMK/CAMKL/CHK1 protein kinase Chk1 ELISA Kit, checkpoint kinase 1 S homeolog ELISA Kit, CHEK1 ELISA Kit, ANI_1_2436074 ELISA Kit, AOR_1_768154 ELISA Kit, PTRG_04183 ELISA Kit, SJAG_01680 ELISA Kit, PAAG_04978 ELISA Kit, MCYG_03290 ELISA Kit, VDBG_03742 ELISA Kit, chek1.S ELISA Kit, Chek1 ELISA Kit, chek1 ELISA Kit
    Gen-ID
    1925
    UniProt
    O14757
    Pathways
    p53 Signalweg, Apoptose, Zellzyklus, DNA Reparatur
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