Bcl-2 CLIA Kit

Produktnummer ABIN5652139

Produktdetails Bcl-2 CLIA Kit

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Reaktivität: Human

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 4.69 ng/mL - 300 ng/mL

Untere Nachweisgrenze: 4.69 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of B-Cell Leukemia/Lymphoma 2 (Bcl2). No significant cross-reactivity or interference between B-Cell Leukemia/Lymphoma 2 (Bcl2) and analogues was observed.

Sensitivität: 0.17 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 - 3 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with an antibody specific to B-Cell Leukemia/Lymphoma 2 (Bcl2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to B-Cell Leukemia/Lymphoma 2 (Bcl2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the B-Cell Leukemia/Lymphoma 2 (Bcl2) level in the sample or standard.,

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level B-Cell Leukemia/Lymphoma 2 (Bcl2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level B-Cell Leukemia/Lymphoma 2 (Bcl2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Andere Bezeichnung: B-Cell Leukemia/Lymphoma 2 (BCL2 Produkte)

Synonyme: Bcl-2 CLIA Kit, PPP1R50 CLIA Kit, bcl-2 CLIA Kit, AW986256 CLIA Kit, C430015F12Rik CLIA Kit, D630044D05Rik CLIA Kit, D830018M01Rik CLIA Kit, BCL-2 CLIA Kit, Bcl2 CLIA Kit, BCL2, apoptosis regulator CLIA Kit, B cell leukemia/lymphoma 2 CLIA Kit, B-cell CLL/lymphoma 2a CLIA Kit, B-cell CLL/lymphoma 2 protein CLIA Kit, BCL2 CLIA Kit, Bcl2 CLIA Kit, bcl2a CLIA Kit, BCL-2 CLIA Kit

Hintergrund:

Gene Name: B-Cell Leukemia/Lymphoma 2

Gene Aliases: B-Cell CLL/Lymphoma 2

Gen-ID: 596

UniProt: P10415

Pathways: MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagie, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling