IL12 ELISA Kit
Kurzübersicht für IL12 ELISA Kit (ABIN5026962)
Target
Alle IL12 ELISA Kits anzeigenReaktivität
Nachweismethode
Methodentyp
Detektionsbereich
Applikation
Proben
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Untere Nachweisgrenze
- 15.6 pg/mL
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Verwendungszweck
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology.
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Analytische Methode
- Quantitative
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Sensitivität
- 9.4 pg/mL
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Produktmerkmale
- This immunoassay kit allows for the in vitro quantitative determination of Human IL-12 (p70) concentrations in serum, plasma. Detection Range: 15.6 - 1000 pg/mL Sensitivity: < 9.4 pg/mL
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Bestandteile
- plate, standard, diluent, antibodies
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Benötigtes Material
- pipettes, tubes, reader, incubator
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Interleukin 12 (IL12) ELISA Kit
IL12 Reaktivität: Huhn Colorimetric Competition ELISA 0.78 pg/mL - 200 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitIL12 Reaktivität: Kaninchen Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitIL12 Reaktivität: Ratte Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 12 (IL12) ELISA KitIL12 Reaktivität: Schwein Colorimetric Sandwich ELISA 0.45 ng/mL - 30 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitIL12 Reaktivität: Hund Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitIL12 Reaktivität: Meerschweinchen Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitIL12 Reaktivität: Human Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitIL12 Reaktivität: Affe Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitIL12 Reaktivität: Ratte Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitIL12 Reaktivität: Schaf Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
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Probenmenge
- 100 μL
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Testdauer
- 3 - 4 h
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Plattentyp
- Pre-coated
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Protokoll
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human IL-12 (p70) antibody was pre-coated into 96-well plates. Biotin conjugated anti-human IL-12 (p70) detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL-12 (p70) amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450 nm in a microplate reader. Concentration of Human IL-12 (p70) can be calculated using the standard curve.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Vorsichtsmaßnahmen
- protect your eyes
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Lagerung
- 4 °C
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Informationen zur Lagerung
- Please refer to the Manual
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- IL12 (Interleukin 12 (IL12))
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Andere Bezeichnung
- IL-12 (p70) (Interleukin 12, p70)
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Pathways
- JAK-STAT Signalweg, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
Target Alle IL12 ELISA Kits anzeigen
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