MAP2K6 ELISA Kit

Produktnummer ABIN4881293

Produktdetails MAP2K6 ELISA Kit

Target: MAP2K6 (Mitogen-Activated Protein Kinase Kinase 6 (MAP2K6))

Bindungsspezifität: pSer207

Reaktivität: Human, Maus

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Verwendungszweck: Cell-Based Human/Mouse MKK6 (S207) Phosphorylation ELISA Kit. Suitable for adherent whole cell lines.

Proben: Cell Culture Cells

Analytische Methode: Semi-Quantitative

Spezifität: The antibodies provided in this kit recognizes human/mouse/rat MKK6 phosphorylated at Serine-207 and total MMK6 for comparison

Produktmerkmale:

• Site and signal pathway-specific
• In vitro detection of adherent cell culture
• No sample lysis needed
• Compatible with a standard ELISA plate reader
• Faster results than with ELISA
• Adaptable for high-throughput screening and drug discovery

Bestandteile:

• uncoated 96-well Microplate
• Wash Buffer A
• Wash Buffer B
• Fixing Solution
• Quenching Buffer
• Blocking Buffer
• Anti-phospho antibody
• Anti-pan antibody
• HRP-Conjugated Secondary Antibody
• TMB One-Step Substrate
• Stop Solution

Benötigtes Material:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Uncoated

Protokoll:

1. Seed 10,000-30,000 cells into each well and incubate overnight.
2. Apply various treatment, inhibitors or activators according to manufacture's instructions.
3. Add 100 μL of Fixing Solution into each well and incubate for 20 min at RT with shaking.
4. Add 200 μL of prepared 1X Quenching Buffer and incubate 20 min at RT.
5. Add 200 μL of Blocking Solution and incubate for 1 h at 37 °C.
6. Add 50 μL of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.
7. Add 50 μL of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 6 months

Details zu MAP2K6

Target: MAP2K6 (Mitogen-Activated Protein Kinase Kinase 6 (MAP2K6))

Andere Bezeichnung: MKK6 (MAP2K6 Produkte)

Synonyme: MAPKK6 ELISA Kit, MEK6 ELISA Kit, MKK6 ELISA Kit, PRKMK6 ELISA Kit, SAPKK-3 ELISA Kit, SAPKK3 ELISA Kit, Mkk6 ELISA Kit, Prkmk6 ELISA Kit, MKK3 ELISA Kit, map2k3 ELISA Kit, zMKK3 ELISA Kit, map2k6 ELISA Kit, mitogen-activated protein kinase kinase 6 ELISA Kit, mitogen-activated protein kinase kinase 6 L homeolog ELISA Kit, MAP2K6 ELISA Kit, Map2k6 ELISA Kit, map2k6 ELISA Kit, map2k6.L ELISA Kit

Gen-ID: 5608

UniProt: P52564

Pathways: MAPK Signalweg, TLR Signalweg, Activation of Innate immune Response, Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades