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MAP2K6 ELISA Kit

MAP2K6 Reaktivität: Human, Maus, Ratte pSer207 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Produktnummer ABIN2748366
  • Target Alle MAP2K6 ELISA Kits anzeigen
    MAP2K6 (Mitogen-Activated Protein Kinase Kinase 6 (MAP2K6))
    Bindungsspezifität
    pSer207, total
    Reaktivität
    • 5
    • 4
    • 2
    • 1
    Human, Maus, Ratte
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human/Mouse/Rat Phospho-MKK6 (S207) ELISA and Total MKK6 ELISA Kit. This assay semi-quantitatively measures phophorylated MKK6 (Ser207) and Total MKK6 in lysate samples.
    Proben
    Cell Lysate, Tissue Lysate
    Analytische Methode
    Semi-Quantitative
    Spezifität
    The antibody pair provided in this kit recognizes human, mouse, and rat MKK6 phosphorylated at site Serine-207 as well as total MKK6.
    Produktmerkmale
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Benötigtes Material
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Top Product
    Discover our top product MAP2K6 ELISA Kit
  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Testdurchführung

    Prepare all reagents and samples as instructed in the manual.
    Add 100 μL of sample or positive control to each well.
    Incubate 2.5 h at RT or O/N at 4 °C.
    Add 100 μL of prepared primary antibody to each well.
    Incubate 1 h at RT.
    Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    Incubate 1 h at RT.
    Add 100 μL of TMB One-Step Substrate Reagent to each well.
    Incubate 30 min at RT.
    Add 50 μL of Stop Solution to each well.
    Read at 450 nm immediately.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Haltbarkeit
    6 months
  • Target Alle MAP2K6 ELISA Kits anzeigen
    MAP2K6 (Mitogen-Activated Protein Kinase Kinase 6 (MAP2K6))
    Andere Bezeichnung
    MKK6 (MAP2K6 Produkte)
    Synonyme
    MAPKK6 ELISA Kit, MEK6 ELISA Kit, MKK6 ELISA Kit, PRKMK6 ELISA Kit, SAPKK-3 ELISA Kit, SAPKK3 ELISA Kit, Mkk6 ELISA Kit, Prkmk6 ELISA Kit, MKK3 ELISA Kit, map2k3 ELISA Kit, zMKK3 ELISA Kit, map2k6 ELISA Kit, mitogen-activated protein kinase kinase 6 ELISA Kit, mitogen-activated protein kinase kinase 6 L homeolog ELISA Kit, MAP2K6 ELISA Kit, Map2k6 ELISA Kit, map2k6 ELISA Kit, map2k6.L ELISA Kit
    Hintergrund
    Dual specificity mitogen-activated protein kinase kinase 6, MAP kinase kinase 6 (MAPKK 6), MAPK/ERK kinase 6
    Gen-ID
    5608
    UniProt
    P52564
    Pathways
    MAPK Signalweg, TLR Signalweg, Activation of Innate immune Response, Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades
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