MAPK14 ELISA Kit

Produktnummer ABIN2950156

Dieses Maus MAPK14 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Maus MAPK14 zu quantifizieren.

Kurzübersicht für MAPK14 ELISA Kit (ABIN2950156)

Target: MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.25 ng/mL - 80 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Produktdetails MAPK14 ELISA Kit

Untere Nachweisgrenze: 1.25 ng/mL

Verwendungszweck: Mouse Mitogen-Activated Protein Kinase 14 (MAPK14) ELISA Kit

Analytische Methode: Quantitative

Sensitivität: 0.75 ng/mL

Bestandteile: The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

• Pre-coated 96-Well Microplate
• Standard
• Standard Diluent Buffer
• Wash Buffer
• Detection Reagent A
• Detection Reagent B
• Diluent A
• Diluent B
• TMB Substrate
• Stop Solution
• Plate Sealer

Benötigtes Material:

• 37 °C incubator
• Multi and single channel pipettes and sterile pipette tips
• Squirt bottle or automated microplate washer
• 1.5 mL tubes
• Distilled water
• Absorbent filter papers
• 100 mL and 1 liter graduated cylinders
• Microplate reader (wavelength: 450 nm)
• ELISA Shaker

Anwendungsinformationen

Applikationshinweise: Optimal dilutions/concentrations should be determined by the end user.

Kommentare:

The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5 % within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.

Plattentyp: Pre-coated

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of MAPK14 were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of MAPK14 were tested on 3 different plates, 8 replicates in each plate.

CV (%) = (Standard Deviation / mean) x 100

Intra-Assay: CV<10%

Inter-Assay: CV<10%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.

Haltbarkeit: 6 months

Details zu MAPK14

Target: MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))

Andere Bezeichnung: Mitogen-Activated Protein Kinase 14

Hintergrund:

Ensembl: ENSMUST00000004990 [P47811-3], ENSMUST00000062694 [P47811-1], ENSMUST00000114752 [P47811-4], ENSMUST00000114754 [P47811-4], ENSMUST00000114758 [P47811-2]

UniProt Entry Name: MK14_MOUSE

Gen-ID: 26416

NCBI Accession: NP_036081

UniProt: P47811

Pathways: MAPK Signalweg, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Hepatitis C, Toll-Like Receptors Cascades, Autophagie, Thromboxane A2 Receptor Signaling, BCR Signaling, S100 Proteine