IL12 ELISA Kit
Kurzübersicht für IL12 ELISA Kit (ABIN2648768)
Target
Alle IL12 ELISA Kits anzeigenReaktivität
Nachweismethode
Applikation
Proben
-
-
Verwendungszweck
- Mouse Interleukin 12p70 (IL-12p70) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of mouse Interleukin 12 (IL-12p70) concentrations in cell culture supernates, serum, and plasma.
-
Analytische Methode
- Quantitative
-
Sensitivität
- 7 pg/ml
-
Produktmerkmale
- Mouse Interleukin 12p70 (IL-12p70) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
-
-
Interleukin 12 (IL12) ELISA Kit
IL12 Reaktivität: Maus Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 12 (IL12) ELISA KitIL12 Reaktivität: Ratte Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 12 (IL12) ELISA KitIL12 Reaktivität: Kaninchen Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitIL12 Reaktivität: Huhn Colorimetric Competition ELISA 0.78 pg/mL - 200 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitIL12 Reaktivität: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitIL12 Reaktivität: Maus Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitIL12 Reaktivität: Ratte Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitHigh Sensitivity IL12 Reaktivität: Human Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitIL12 Reaktivität: Hamster Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitIL12 Reaktivität: Schaf Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
-
-
Applikationshinweise
- Optimal working dilution should be determined by the investigator.
-
Probenmenge
- 100 μL
-
Testdauer
- 3.5 h
-
Plattentyp
- Pre-coated
-
Protokoll
- Mouse Interleukin 12p70 (IL-12p70) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-12p70 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-12p70 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-12p70 is added to the wells and binds to the combination of capture antibody- IL-12p70 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-12p70 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-12p70 standard dilutions and IL-12p70 sample concentration determined.
-
Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
-
-
Lagerung
- 4 °C
-
-
- IL12 (Interleukin 12 (IL12))
-
Andere Bezeichnung
- IL-12p70
-
Pathways
- JAK-STAT Signalweg, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
Target Alle IL12 ELISA Kits anzeigen
-
