CHEK1 ELISA Kit

Produktnummer ABIN2043125

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Maus CHEK1.

Kurzübersicht für CHEK1 ELISA Kit (ABIN2043125)

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails CHEK1 ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Marke: BioAssay™

Sensitivität: < 0.058 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Checkpoint Homolog (CHEK1) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Checkpoint Homolog (CHEK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Checkpoint Homolog (CHEK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Checkpoint Homolog (CHEK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Checkpoint Homolog (CHEK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu CHEK1

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Andere Bezeichnung: Checkpoint Homolog (CHEK1)

Pathways: p53 Signalweg, Apoptose, Zellzyklus, DNA Reparatur