IL12 ELISA Kit

Produktnummer ABIN2042522

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Maus IL12.

Kurzübersicht für IL12 ELISA Kit (ABIN2042522)

Target: IL12 (Interleukin 12 (IL12))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6-1000 pg/mL

Applikation: ELISA

Produktdetails IL12 ELISA Kit

Untere Nachweisgrenze: 15.6 pg/mL

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 1.5 pg/mL

Bestandteile: 96-well plate pre-coated with anti-IL-12(p70), Mouse
Lyophilized recombinant IL-12(p70), Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Sandwich ELISA quantitative detection kit for Mouse IL-12(p70) in cell culture supernates and serum.Interleukin (IL)-12 is a 70kD cytokine comprised of two disulfide-linked proteins (p35 and p40) and is essential for the initiation of effective immune response. And the IL-12p70 is a heterodimer of p35 and p40 subunits, it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation. Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40 (protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70. The standard product used in this kit is recombinant mouse IL-12 p70 comprising of a 193 amino acids p35 chain and a 313 amino acids p40 chain connected by a disulfide bond.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu IL12

Target: IL12 (Interleukin 12 (IL12))

Andere Bezeichnung: IL-12(p70)

Gen-ID: 16159

UniProt: F8WI71

Pathways: JAK-STAT Signalweg, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome