GSK3 alpha Antikörper (pSer21)

Produktnummer ABIN99927

Hersteller Produkt- Nr.: 600-401-429

Produktdetails anti-GSK3 alpha Antikörper

Target: GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))

Bindungsspezifität: pSer21

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GSK3 alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

Hersteller Produkt- Nr.: 600-401-429

Hersteller: Rockland

Verwendungszweck: GSK3 Alpha phospho S21 Antibody

Kreuzreaktivität (Details): This affinity-purified antibody is directed against the phosphorylated form of human GSK3A at the pS 21 residue.

Produktmerkmale: Synonyms: rabbit anti-GSK3 alpha pS21 antibody, rabbit anti-GSK3α pS21 antibody, GSK 3 alpha pS21 antibody, Glycogen synthase kinase-3 alpha antibody, phosphorylated GSK-3 alpha antibody, GSK 3A antibody, GSK-3A antibody, GSK3alpha antibody, Serine/threonine-protein kinase GSK3A

Aufreinigung: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N-Terminal region near aa 1-25 of human GSK3 alpha.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: This affinity-purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 52 kDa in size corresponding to GSK3A by western blotting in the appropriate cell lysate or extract.  Less than 1 % reactivity is observed against the non-phosphorylated form of the immunizing peptide.  This antibody is phospho specific for pS21 of GSK3A.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:2,000 - 1:10,000

IF Microscopy Dilution: User Optimized

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.28 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu GSK3 alpha

Target: GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))

Andere Bezeichnung: GSK3A (GSK3a Produkte)

Hintergrund: Background: Glycogen synthase kinase 3 alpha (GSK3A) belongs to the ser/thr family of protein kinases, Cdc2/cdkx subfamily, gsk-3 subsubfamily. It is implicated in the hormonal control of several regulatory proteins including glycogen synthase, myb, and the transcription factor c-jun.  GSK3A is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. Two isoforms, alpha (GSK3A) and beta (GSK3B), show a high degree of amino acid homology. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.

Gen-ID: 2931, 49574532

UniProt: P49840

Pathways: PI3K-Akt Signalweg, WNT Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, BCR Signaling, Warburg Effekt