Caspase 8 Antikörper

Produktnummer ABIN969003

Dieses Anti-Caspase 8-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Caspase 8 in WB, IHC, ELISA und FACS. Geeignet für Human, Maus, Ratte und Affe. Dieses Primary Antibody wurde in 2+ Publikationen zitiert.

Kurzübersicht für Caspase 8 Antikörper (ABIN969003)

Target: Caspase 8 (CASP8)

Reaktivität: Human, Maus, Ratte, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Caspase 8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Klon: 1H11

Produktdetails anti-Caspase 8 Antikörper

Verwendungszweck: CASP8 Antibody

Aufreinigung: Ascitic fluid

Immunogen: Purified recombinant fragment of human CASP8 expressed in E. Coli.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

ELISA: 1/10000

FCM: 1/200 - 1/400

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Referenzen für anti-Caspase 8 Antikörper (ABIN969003)

Fulda: "Caspase-8 in cancer biology and therapy." in: Cancer letters, Vol. 281, Issue 2, pp. 128-33, (2009) (PubMed).

Jani, Gobejishvili, Hote, Barve, Joshi-Barve, Kharebava, Suttles, Chen, McClain, Barve: "Inhibition of methionine adenosyltransferase II induces FasL expression, Fas-DISC formation and caspase-8-dependent apoptotic death in T leukemic cells." in: Cell research, Vol. 19, Issue 3, pp. 358-69, (2009) (PubMed).

Details zu Caspase 8

Target: Caspase 8 (CASP8)

Andere Bezeichnung: CASP8

Hintergrund: This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.

Molekulargewicht: 26 kDa

Gen-ID: 841

UniProt: Q14790

Pathways: Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades