MAPK14 Antikörper (AA 243-355)

Produktnummer ABIN968770

Produktdetails anti-MAPK14 Antikörper

Target: MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))

Bindungsspezifität: AA 243-355

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MAPK14 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Kreuzreaktivität: Hund, Maus, Ratte (Rattus)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human p38 MAPK aa. 243-355

Klon: 27-p38alpha-SAPK2a

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968537, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-MAPK14 Antikörper (ABIN968770)

Morinobu, Gadina, Strober, Visconti, Fornace, Montagna, Feldman, Nishikomori, OShea: "STAT4 serine phosphorylation is critical for IL-12-induced IFN-gamma production but not for cell proliferation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 19, pp. 12281-6, (2002) (PubMed).

Winston, Chan, Johnson, Riches: "Activation of p38mapk, MKK3, and MKK4 by TNF-alpha in mouse bone marrow-derived macrophages." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 9, pp. 4491-7, (1997) (PubMed).

Brunet, Pouysségur: "Identification of MAP kinase domains by redirecting stress signals into growth factor responses." in: Science (New York, N.Y.), Vol. 272, Issue 5268, pp. 1652-5, (1996) (PubMed).

Han, Lee, Bibbs, Ulevitch: "A MAP kinase targeted by endotoxin and hyperosmolarity in mammalian cells." in: Science (New York, N.Y.), Vol. 265, Issue 5173, pp. 808-11, (1994) (PubMed).

Details zu MAPK14

Target: MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))

Andere Bezeichnung: p38alpha (MAPK14 Produkte)

Hintergrund: Activation of the immune and inflammatory responses often involves the recognition of bacterial endotoxin (lipopolysaccharide or LPS). Binding of LPS by monocytic cells results in the production and release of proinflammatory cytokines, such as IL-1 and TNF-alpha. LPS-induced signaling cascades involve members of the Ser/Thr protein kinase family known as the mitogen activated protein kinases (MAPKs). One of these, p38 MAP kinase (p38 MAPK or CSPB2), is a human homologue of HOG1, a yeast MAP kinase that is essential for growth under conditions of elevated osmolarity. Similar to HOG1, p38 MAPK is phosphorylated in response to hyperosmolarity, but also in response to a variety of stimuli including LPS, IL-1, or TNF-alpha. Efficient activation of p38 MAPK requires phosphorylation of Thr-180 and Tyr-182. At least three Thr/Tyr kinases (MKK3, MKK4/SEK1, and MKK6) phosphorylate and activate p38 MAPK. This leads to the activation of multiple transcription factors (NF-kappaB, ATF-2, Elk-1, and CHOP/GADD143) that induce expression of proinflammatory cytokine genes. In addition to its role in immunity, p38 MAPK is widely expressed and serves as an important signal transducer in a variety of cell types. This antibody is routinely tested by western blot analysis.
Synonyms: SAPK2a

Molekulargewicht: 42 kDa

Pathways: MAPK Signalweg, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Hepatitis C, Toll-Like Receptors Cascades, Autophagie, Thromboxane A2 Receptor Signaling, BCR Signaling, S100 Proteine