CD40 Antikörper (AA 71-187)

Produktnummer ABIN968495

Der Maus Monoklonal Anti-CD40-Antikörper wurde für WB und IF validiert. Er ist geeignet, CD40 in Proben von Human zu detektieren. Es sind 3+ Publikationen verfügbar.

Kurzübersicht für CD40 Antikörper (AA 71-187) (ABIN968495)

Target: CD40

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD40 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Klon: 41-CD40

Produktdetails anti-CD40 Antikörper

Bindungsspezifität: AA 71-187

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human CD40 aa. 71-187

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968584, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-CD40 Antikörper (ABIN968495)

Morio, Hanissian, Bacharier, Teraoka, Nonoyama, Seki, Kondo, Nakano, Lee, Geha, Yata: "Ku in the cytoplasm associates with CD40 in human B cells and translocates into the nucleus following incubation with IL-4 and anti-CD40 mAb." in: Immunity, Vol. 11, Issue 3, pp. 339-48, (1999) (PubMed).

Nguyen, Duncan, Mirtsos, Ng, Speiser, Shahinian, Marino, Mak, Ohashi, Yeh: "TRAF2 deficiency results in hyperactivity of certain TNFR1 signals and impairment of CD40-mediated responses." in: Immunity, Vol. 11, Issue 3, pp. 379-89, (1999) (PubMed).

Randall, Heath, Santos-Argumedo, Howard, Weissman, Lund: "Arrest of B lymphocyte terminal differentiation by CD40 signaling: mechanism for lack of antibody-secreting cells in germinal centers." in: Immunity, Vol. 8, Issue 6, pp. 733-42, (1998) (PubMed).

Details zu CD40

Target: CD40

Andere Bezeichnung: CD40

Hintergrund: CD40, a member of the TNF receptor family, contains a cysteine-rich N-terminal domain and a Ser/Thr-rich region preceding the transmembrane domain. On B cells, signaling through CD40 induces cell growth and differentiation, mediates cell survival within the germinal center, and upregulates the expression of costimulatory and adhesion molecules, such as B7.1, B7.2, and ICAM-1. The interaction of CD40 on B cells and CD40L on activated CD4+ T cells is essential for immune functions, such as immuoglobulin class switching. Signal transduction through CD40 pathways involves interaction with proteins such as TRAFs (TRAF2, TRAF3, TRAF5, and TRAF6), Jak 3, and Tyr phosphorylation of proteins, such as Lyn, Syk, PI-3-kinase, STAT3, and STAT5. In TRAF2-deficient mice, CD40-mediated B cell proliferation and NFkappaB activation are defective. Ku70 and Ku80 associate with the membrane-proximal region of CD40 in human primary B cells and the engagement of CD40 leads translocation of Ku proteins to the nucleus. Thus, CD40 interacts with a variety of signal transducers which mediate its role in B cell survival, growth, differentiation, and immunoglobulin class switching.

Molekulargewicht: 44 kDa

Pathways: NF-kappaB Signalweg, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints