Cyclin A Antikörper (AA 26-144)

Produktnummer ABIN968445

Dieses Anti-Cyclin A-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Cyclin A in WB und BI. Geeignet für Human. Dieses Primary Antibody wurde in 5+ Publikationen zitiert.

Kurzübersicht für Cyclin A Antikörper (AA 26-144) (ABIN968445)

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cyclin A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), BioImaging (BI)

Klon: 25-Cyclin A

Produktdetails anti-Cyclin A Antikörper

Bindungsspezifität: AA 26-144

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
6. Triton is a trademark of the Dow Chemical Company.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Cyclin A aa. 26-144

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Bioimaging
1. Seed the cells in appropriate culture medium at ~10,000 cells per well in an 96-well Imaging Plate and culture overnight.
2. Remove the culture medium from the wells, and fix the cells by adding 100 myl of Fixation Buffer to each well. Incubate for 10 minutes at room temperature (RT).
3. Remove the fixative from the wells, and permeabilize the cells using either 90% methanol, or Triton™ X-100: a. Add 100 myl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. OR b. Add 100 myl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT.
4. Remove the permeabilization buffer, and wash the wells twice with 100 myl of 1× PBS.
5. Remove the PBS, and block the cells by adding 100 myl of to each well. Incubate for 30 minutes at RT.
6. Remove the blocking buffer and add 50 myl of the optimally titrated primary antibody (diluted in Stain Buffer) to each well, and incubate for 1 hour at RT.
7. Remove the primary antibody, and wash the wells three times with 100 myl of 1× PBS.
8. Remove the PBS, and add the second step reagent at its optimally titrated concentration in 50 myl to each well, and incubate in the dark for 1 hour at RT.
9. Remove the second step reagent, and wash the wells three times with 100 myl of 1× PBS.
10. Remove the PBS, and counter-stain the nuclei by adding 200 myl per well of 2 myg/ml Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
11. View and analyze the cells on an appropriate imaging instrument.

Kommentare:

Related Products: ABIN967389, ABIN968533

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-Cyclin A Antikörper (ABIN968445)

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, (2002) (PubMed).

Henglein, Chenivesse, Wang, Eick, Bréchot: "Structure and cell cycle-regulated transcription of the human cyclin A gene." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 12, pp. 5490-4, (1994) (PubMed).

Pines, Hunter: "The differential localization of human cyclins A and B is due to a cytoplasmic retention signal in cyclin B." in: The EMBO journal, Vol. 13, Issue 16, pp. 3772-81, (1994) (PubMed).

Pines: "Cyclins and cyclin-dependent kinases: take your partners." in: Trends in biochemical sciences, Vol. 18, Issue 6, pp. 195-7, (1993) (PubMed).

Giordano, Whyte, Harlow, Franza, Beach, Draetta: "A 60 kd cdc2-associated polypeptide complexes with the E1A proteins in adenovirus-infected cells." in: Cell, Vol. 58, Issue 5, pp. 981-90, (1989) (PubMed).

Details zu Cyclin A

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Andere Bezeichnung: Cyclin A

Hintergrund: Progression of the mammalian cell cycle is regulated by phosphorylation of many key proteins. Several classes of cyclins (A-E) act as regulatory subunits for cyclin-dependent kinases (cdks). These cyclin-cdk holoenzymes are essential for proper control of cell cycle progression. They phosphorylate and regulate a variety of substrates whose activity is required for cell cycle transitions. The temporal expression of cyclins is tightly regulated throughout the cell cycle by synthesis and degradation. Such regulation plays a critical role in controlling the enzymatic activity of the cdks. Cyclin A, one of the mitotic cyclins, activates Cdk2 near the start of S phase and is necessary for the initiation of DNA replication. In mammalian somatic cells, Cyclin A is required during S phase and passage through G2. The D and E type cyclins regulate passage through G1, while Cyclin B is a critical regulator of mitosis. It has been shown in a number of species that mutation or disruption of normal Cyclin A expression causes cells to arrest at G2. Cyclin A binds both the cdc2 (Cdk1) and Cdk2 kinases and may also have a role in mitotic dependence on S phase completion.

Molekulargewicht: 60 kDa

Pathways: PI3K-Akt Signalweg, Zellzyklus, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA