TNF alpha Antikörper

Produktnummer ABIN967569

Zitiert in 4+ Publikationen. Der Armenischer Hamster Monoklonal anti-TNF alpha Antikörper (Klon TN3-19-12) (ABIN967569) detektiert spezifisch TNF alpha in WB, ELISA, IP und Neut. Dieser Antikörper reagiert spezifisch mit Proben aus Maus.

Kurzübersicht für TNF alpha Antikörper (ABIN967569)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reaktivität: Maus

Wirt: Armenischer Hamster

Klonalität: Monoklonal

Konjugat: Dieser TNF alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Neutralization (Neut)

Klon: TN3-19-12

Produktdetails anti-TNF alpha Antikörper

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Recombinant mouse TNF protein

Isotyp: IgG1 lambda

Anwendungsinformationen

Applikationshinweise: 1. Neutralization:
This monoclonal antibody is useful to neutralize the bioactivity of rat TNF as tested on L929 cells at 2 x 10^5 cells/ml in cytolysis assay measured by MTT, using 12 ng/ml recombinant mouse TNF and preincubation of antibody and ligand = 60 minutes.

2. ELISA:
The purified TN3-19.12 antibody can be used as a capture antibody for a sandwich ELISA that measures TNF protein levels. For measuring mouse or rat TNF in complex fluids such as serum, ELISA Kits are recommended (refer to website).

3. IP/WB:
The TN3-19.12 antibody has been reported to be useful for immunoprecipitation and Western blotting. Please note that this application is not routinely tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Konservierungsmittel: Azide free

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Referenzen für anti-TNF alpha Antikörper (ABIN967569)

Takahashi, Kapás, Fang, Krueger: "An anti-tumor necrosis factor antibody suppresses sleep in rats and rabbits." in: Brain research, Vol. 690, Issue 2, pp. 241-4, (1996) (PubMed).

Ferran, Dautry, Mérite, Sheehan, Schreiber, Grau, Bach, Chatenoud: "Anti-tumor necrosis factor modulates anti-CD3-triggered T cell cytokine gene expression in vivo." in: The Journal of clinical investigation, Vol. 93, Issue 5, pp. 2189-96, (1994) (PubMed).

Leiby, Fortier, Crawford, Schreiber, Nacy: "In vivo modulation of the murine immune response to Francisella tularensis LVS by administration of anticytokine antibodies." in: Infection and immunity, Vol. 60, Issue 1, pp. 84-9, (1992) (PubMed).

Sheehan, Ruddle, Schreiber: "Generation and characterization of hamster monoclonal antibodies that neutralize murine tumor necrosis factors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 11, pp. 3884-93, (1989) (PubMed).

Details zu TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Andere Bezeichnung: TNF

Hintergrund: The TN3-19.12 antibody reacts with rat and mouse tumor-necrosis factor (TNF) proteins (also known as TNF-alpha) . Moreover, the TN3-19.12 antibody is reported to crossreact with rabbit TNF, but it does not crossreact with mouse lymphotoxin-alpha (LT-alpha, also known as TNF-beta) nor with human TNF. The immunogen used to generate the TN3-19.12 hybridoma was E. coli-expressed, purified recombinant mouse TNF protein. This monoclonal antibody has been reported to neutralize the bioactivities of mouse, rat and rabbit TNF.

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome