FADD Antikörper

Produktnummer ABIN967524

Dieses Anti-FADD-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von FADD in WB und IP. Geeignet für Human. Dieses Primary Antibody wurde in 2+ Publikationen zitiert.

Kurzübersicht für FADD Antikörper (ABIN967524)

Target: FADD (Fas (TNFRSF6)-Associated Via Death Domain (FADD))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FADD Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Klon: A66-2

Produktdetails anti-FADD Antikörper

Marke: BD Pharmingen™

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human FADD GST

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Clone A66-2 can be used for western blot analysis (1-2 µg/ml). Other reported applications not routinely tested include immunoprecipitation (1-2 µg/1x10^6 cells). Daudi B lymphoma cells (ATCC CCL-213) are suggested as a positive control.

Kommentare:

Related Products: ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-FADD Antikörper (ABIN967524)

Muzio, Chinnaiyan, Kischkel, ORourke, Shevchenko, Ni, Scaffidi, Bretz, Zhang, Gentz, Mann, Krammer, Peter, Dixit: "FLICE, a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death--inducing signaling complex." in: Cell, Vol. 85, Issue 6, pp. 817-27, (1996) (PubMed).

Cleveland, Ihle: "Contenders in FasL/TNF death signaling." in: Cell, Vol. 81, Issue 4, pp. 479-82, (1995) (PubMed).

Details zu FADD

Target: FADD (Fas (TNFRSF6)-Associated Via Death Domain (FADD))

Andere Bezeichnung: FADD

Hintergrund: FADD is a molecule involved in the Fas-mediated cell death pathway. Apoptosis is induced when Fas ligand or agonistic Fas antibodies bind to the Fas receptor, and trigger the activation of a cell death signaling pathway. Induction of Fas-mediated apoptosis requires a conserved cytoplasmic motif, referred to as the death domain, that is present in the C-terminal end of Fas. FADD also contains a death domain, and Fas and FADD bind to each other through their respective death domains. Death domains are thought to act as adaptor proteins by linking Fas and other members of the tumor necrosis factor receptor (TNFR) superfamily to downstream signaling pathways. Overexpression of FADD in vitro leads to cell death suggesting that FADD, like FAS, is an apoptosis-inducing protein. The N-terminal, but not the C-terminal death domain, is required for apoptosis induced by FADD overexpression. It is thought that the amino-terminal region of FADD functions by binding to caspase-3 and thereby linking signals from the cell surface to an apoptopic protease cascade. FADD has a calculated molecular weight of 24 kDa and migrates at a molecular weight of ~27 kDa in SDS/PAGE.

Molekulargewicht: 24-27 kDa

Pathways: Apoptose, TLR Signalweg, Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades