CD11b Antikörper

Produktnummer ABIN967477

Dieses Maus Monoklonal-Antikörper erkennt spezifisch CD11b in FACS, WB, IP, IHC (fro) und BR. Er zeigt eine Reaktivität gegenüber Ratte und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für CD11b Antikörper (ABIN967477)

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD11b Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR)

Klon: WT-5

Produktdetails anti-CD11b Antikörper

Marke: BD Pharmingen™

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat neutrophils

Isotyp: IgA kappa

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4° C.

Referenzen für anti-CD11b Antikörper (ABIN967477)

Watanabe, Arakawa, Fukuda, Higuchi, Kobayashi: "Role of neutrophils in a rat model of gastric ulcer recurrence caused by interleukin-1 beta." in: The American journal of pathology, Vol. 150, Issue 3, pp. 971-9, (1997) (PubMed).

Tamatani, Kitamura, Kuida, Shirao, Mochizuki, Suematsu, Schmid-Schönbein, Watanabe, Tsurufuji, Miyasaka: "Characterization of rat LECAM-1 (L-selectin) by the use of monoclonal antibodies and evidence for the presence of soluble LECAM-1 in rat sera." in: European journal of immunology, Vol. 23, Issue 9, pp. 2181-8, (1993) (PubMed).

Details zu CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Andere Bezeichnung: CD11b

Hintergrund: The WT.5 antibody reacts with the alpha subunit of Mac-1 (alphaMbeta2, CD11b/CD18) found on neutrophils and myeloid cells, but not on lymphocytes. Mac-1 expression is rapidly upregulated on neutrophils after activation, in the same time period that CD62L (L-selectin) is shed from the cell surface. Mac-1 mediates adhesion to ICAM-1 (CD54) and C3bi. Neutrophil binding to ICAM-1 is blocked by mAb WT.5. This antibody is routinely tested by flow cytometric analysis.
Synonyms: Integrin alphaM chain, Mac-1 alpha chain

Pathways: Apoptose, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation